Imaginal disc ab staining

Cathy Slack cathy.slack at ic.ac.uk
Tue Jul 17 09:57:37 EST 2001


I am trying to stain drosophila third instar imaginal discs (wing) using
an anti-achaete antibody and HRP-DAB staining. However I either cannot
get any signal at all or too much background so that the staining
pattern can't be made out! This is trying different fixation methods. I
have tried 4% formaldehyde for various times and temperatures down to
1%. All of my reagents are ok so it must be my method. Can anyone
help????

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