S2 transfections

Olivier BLARD ol_blard at club-internet.fr
Thu Sep 20 08:16:46 EST 2001


Hello,
In our lab, calcium phosphate works better than lipofectine and fugene6
on S2 cells, but the ratio of transfected cells (using GFP) is not very
good (maybe 1/30). I think DOTAP is good but we have not received this
product yet.

--

Olivier.

Louisa Flintoft <louisa.flintoft at kcl.ac.uk> a écrit dans le message :
a05010402b7ce4e4c1f33@[159.92.52.25]...
> Can anyone help me with my S2 problems? I've been transfecting using
> calcium phosphate, and I've had very mixed results in terms of
> efficiency of transfection, cell death etc. Has anyone used this
> method and got it to work consistently well? Alternatively, can
> anyone recommend an alternative method that works well?
>
> Thanks,
>
> Louisa FLintoft
>
> --
> Dr Louisa Flintoft
>
> Department of Molecular Neurobiology
> 4th Floor
> New Hunt's House
> Guy's Campus
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> London Bridge
> London SE1 9RT
>
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