homogenization of Drosophila larvae?

Bregje Wertheim b.wertheim at ucl.ac.uk
Mon Apr 15 11:54:04 EST 2002


Hi,

For my RNA extractions, I have troubles with homogenizing early second 
instar larvae. Larger larvae can be easily homogenized with either 
(motorized) pestle or bead mills, but the small larvae seem to slip 
through. Has anyone some good advise how to grind up these tiny larvae? I 
need to homogenize groups of approx. 50-100 larvae, without loss of RNA and 
without cross contamination between samples. Hope you can help me out!
Regards,


Bregje Wertheim
Department of Biology
University College London
UK
b.wertheim at ucl.ac.uk

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