heat fixation dros@net.bio.netros@net.bio.net&replyto=3C7F688A.E63AA0BC@mail.uni-mainz.d e&subject=Re:heat fixation@cmgm.stanford.edu

Stefan Luschnig luschnig at pmgm2.stanford.edu
Tue Mar 26 10:05:30 EST 2002


Hi,

there is a protocol for heat fixation in the "Roberts " methods book 
("Drosophila: a practical approach") in the chapter "looking at 
embryos".
just briefly : you essentially collect embryos in a small steel mesh, 
throw them in boiling 0.1 % NaCl, 0.1% Triton-X 100 for 40 sec (in a 
scintillation vial), add more cold solution, and chill them on ice, 
before you devitellinize them in heptane/methanol as usual.  It 
seems to work well for certain proteins.

good luck,
Stefan

luschnig at cmgm.stanford.edu


-- 
___________________________________________________________
Stefan Luschnig
Department of Biochemistry/HHMI
279 Campus Drive, Beckman Center Rm. B453
Stanford University School of Medicine
Stanford, CA 94305
USA

lab phone	(650)-723-5872
Fax		(650)-723-6783
E-mail		luschnig at cmgm.stanford.edu
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