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Kelly Beumer kelly at biochem.utah.edu
Fri Apr 11 08:35:37 EST 2003


Hi, Tony,
I am thinking about making gateway vectors as well, and saw that 
Terry Murphy at the Carnegie institute has made a few that will be 
available to the community soon. Did you get any input on this note 
you posted last october on flybase?

ref: news.indiana.edu bionet.drosophila:148

Fly gurus,
         We're wondering if there is any experience using p element
transformation vectors (for example pUAST) made by gateway cloning
(invitrogen).   Is there any difference in expression or mobilization
from a p-element having the 20-odd basepair recombination site
internally in the polylinker?  We are making  p-element transformation
plasmids this way, but would like to know if we are getting ourselves
into trouble.

Thanks,

Thanks for lettling me know!

-- 
Kelly Beumer
Biochemistry Department
University of Utah
20 N. 1900 East, Rm. 211
Salt Lake City , UT 84132-3201

1-801-581-3848
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