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Kelly Beumer
kelly at biochem.utah.edu
Fri Mar 14 09:09:38 EST 2003
Hi, Tony,
I am thinking about making gateway vectors as well, and saw that
Terry Murphy at the Carnegie institute has made a few that will be
available to the community soon. Did you get any input on this note
you posted last october on flybase?
ref: news.indiana.edu bionet.drosophila:148
Fly gurus,
We're wondering if there is any experience using p element
transformation vectors (for example pUAST) made by gateway cloning
(invitrogen). Is there any difference in expression or mobilization
from a p-element having the 20-odd basepair recombination site
internally in the polylinker? We are making p-element transformation
plasmids this way, but would like to know if we are getting ourselves
into trouble.
Thanks,
Thanks for lettling me know!
--
Kelly Beumer
Biochemistry Department
University of Utah
20 N. 1900 East, Rm. 211
Salt Lake City , UT 84132-3201
1-801-581-3848
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