Experience of using dsRED in zebrafish
plessy at titus.u-strasbg.fr
Thu Mar 20 08:30:12 EST 2003
I will not answer directly your question :(
I used clontech's nuclear dsRED to get stable zebrafish transgenics.
My analysis are performed in 24-48h old embryos.
It takes about 18h to get some red fluorescence at 28° (compared to 1 - 2 h
The fluorescence can be very strong, so strong that a red signal is visible
using the GFP filter on a Leica binocular.
I didn't see a decent "fluorescent timer" effect : it seems that blue light
can excitate dsRED in some conditions, and that some signal can be read
with "gfp" settings under the confocal. But I never saw green fluorescence
under a binocular scope. With a strong transgene, it looks more like some
I am now very happy with CFP and YFP, that are easy to discriminate, and
that have same maturation speed than GFP.
More information about the Dros