whole embryo TEM techniques and tips

Suniti Naqvi suniti at tll.org.sg
Wed May 14 11:11:09 EST 2003


Hi! I'm very new to Drosophila and would like to look at membrane 
structures in early embryonic stages at the EM level. I would 
appreciate any protocols for sample preparation for TEM (fixative, 
sectioning, staining) that preserve membrane structures (coated pits, 
vesicles, etc.) in very early embryos upto cellularisation.
Thanks very much!
Suniti Naqvi 
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