mariner-containing Drosophila strain

Joana Silva jsilva at tigr.org
Fri Nov 7 12:25:35 EST 2003


Hi,

I am looking for genomic DNA from flies (or the flies themselves) that carry
active members of the Mos mariner transposable element. These would most
likely be strains of D. simulans and of D. mauritiana ( D. melanogaster will
not do).  The genomic DNA is intended for control in PCR reactions using
degenerate primers designed for D,D34D mariners. My questions are as
follows:

- are there any research groups in the Maryland/DC/Virginia area that would
be willing to spare a small amount of genomic DNA (or flies) of these
strains? I will drive by and pick it up.

- In order to extract larger amounts of DNA for Southern blots in the
future, we are thinking of ordering flies from the Drosophila Stock Center.
Will any wild type strain of the above two species carry Mos, or should we
try only European/African strains?

Thanks for any advice.
Joana


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Joana S.C. Carneiro da Silva, Ph.D.

The Institute for Genomic Research
9712 Medical Center Drive
Rockville MD 20850

jsilva at tigr.org  voice:301.838.5896 fax:301.838.0208
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