Help with Lethal Mutations

Chris Jones cjones at moravian.edu
Thu May 20 13:49:38 EST 2004


In article <490D0AFAF3D2D3119F6C00508B6FDF1506965A9D at ex.mshri.on.ca>,
 siddiqui at mshri.on.ca (Najeeb Siddiqui) wrote:

> I am new in working with Drosophila so this might be a naive question to
> some. I have received a piggyback (Exelexis) insertion line (balanced by
> TM6B, Tb[1] ) that is embryonic lethal for the insertion. I am wondering if
> there is an easy way to identify the dead embryos (due to homozygosity of
> the balancer) from the other dead embryos (dead due to homozygosity at the
> insertion locus)? I will greatly appreciate any help in this matter.

Put your insertion chromosome over another balancer with an 
easily-scored marker, such as GFP.

Chris Jones




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