[Drosophila] PEP transposon jumpout mutagenesis
Man.Mohan at gen.bio.uni-giessen.de
Sat Aug 6 15:54:50 EST 2005
I am trying to make a jumpout of a PEP transposon sitting on the 3L (in my
gene). My first trial where I generated about 60 lines white eyed lines
failed to give any imprecise jumpout. Has anyone tried making a imprecise
jumpout of a PEP element. How many lines I should generate to get a
imprecise jumpout. I am interested in deleting a chunk of genomic DNA, which
might give me a better understanding of the functions of the gene, where
this pep is sitting.
Thanks a lot in advance.
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