[Drosophila] Re: Dros Digest, Vol 20, Issue 7

Graham Thomas via dros%40net.bio.net (by gxt5 At psu.edu)
Fri Dec 15 16:55:47 EST 2006



Dear Chrstina,

I'm not sure from your posting what the exact purpose of your oligo's 
is to be - but if its just sequence verification of your clones and 
you can't get the vector sequence you need to design your own - this 
primer works for us: 5'-gCCAAgAAgTAATTATTg-3'

cheers!
Graham

Quoting dros-request At oat.bio.indiana.edu:

>Send Dros mailing list submissions to
>	dros At net.bio.net
>
>To subscribe or unsubscribe via the World Wide Web, visit
>	http://www.bio.net/biomail/listinfo/dros
>or, via email, send a message with subject or body 'help' to
>	dros-request At net.bio.net
>
>You can reach the person managing the list at
>	dros-owner At net.bio.net
>
>When replying, please edit your Subject line so it is more specific
>than "Re: Contents of Dros digest..."
>
>
>Today's Topics:
>
>    1. sequence of the pUAST vector (Christina Wagner)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Fri, 15 Dec 2006 10:55:47 +0100
>From: Christina Wagner <cwagner At zoologie.uni-kiel.de>
>Subject: [Drosophila] sequence of the pUAST vector
>To: dros At magpie.bio.indiana.edu
>Message-ID:
>	<49AF4317-C8A4-4913-B33C-5F9DD648CA36 At zoologie.uni-kiel.de>
>Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
>
>Hallo,
>I would like to generate oligos for the sequences which I ligated
>into the pUAST vector. Does anybody have the vector sequence? The
>sequence of the vector enclosing the multiple cloning side is
>sufficient for the generation of the primers.
>Thank you very much in advance.
>Christina
>
>
>
>------------------------------
>
>_______________________________________________
>Dros mailing list
>Dros At net.bio.net
>http://www.bio.net/biomail/listinfo/dros
>
>End of Dros Digest, Vol 20, Issue 7
>***********************************
>




_______________________________________________
Dros mailing list
Dros At net.bio.net
http://www.bio.net/biomail/listinfo/dros
-- 

Departments of Biology and of Biochemistry & Molecular Biology,
208 Mueller Laboratory,
The Pennsylvania State University,
University Park, PA 16802

Email: gxt5 At psu.edu
Tel: (814) 863-0716
Fax: (814) 865-9131
W3: http://www.bmb.psu.edu/faculty/thomas/lab/default.htm

Please direct any aspiring graduate students interested in the Cell 
and Developmental Biology to  our  interdisciplinary graduate 
program:  http://www.huck.psu.edu/GradEd/CDB/home.html


More information about the Dros mailing list