[bio.dros] [Drosophila] (no subject)

Maeda Tatsuo maeda19721104 at yahoo.co.jp
Thu Feb 23 20:35:21 EST 2006


Hi, Vadali

How about a fixation with 4% formaldehide?

But, a staining of an oviduct is deficult.
I was very suffering it, too.
Hang in there!

My name is Tatsuro Maeda.
>From Tokyo metropolitan UV. Graduated student.

See you.


--- Kavita Vadali <kvadali at ilstu.edu> からのメッセージ:
> Hi,
> I am trying to do immunostaining of fly ovaries with
> anti-crumbs antibody
> (ascites from DSHB) and am failing at it. I have
> tried 1:1000 and 1:500
> concentrations of the antibody but I don't see even
> a faint signal. I want to
> try methanol fixing the ovaries but I don't have the
> protocol for that. Has
> anybody done this fixation on ovaries? Could you
> please send me the detailed
> protocol for that?
> Thanks a lot.
> 
> Kavita Vadali
> 
> -- 
> Kavita Vadali
> PhD Candidate
> Division of Biomedical Sciences,
> Department of Biological Sciences,
> Illinois State University,
> Campus Box 4120,
> Normal, IL 61790-4120
> Phone: (309)438-5075
> Fax: (309)438-3722
> 
> 
> 
> 
> 
>
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