[Drosophila] In situ hybridization

Serena Duchi serena.duchi2 at unibo.it
Thu Jun 8 09:00:16 EST 2006

I've serious problems with in situ hybridization 'cause my RNA probe isn't good labeled. To label the probe I use the Roche's kit SP6/T7 DIG RNA Labeling kit and my probe is 341 bases.
Does anyone have some suggestions to improve the efficiency of labeling and also in situ protocol???
Thank's a lot in advance 

Serena Duchi 
Unit of Developmental genetics
Lab of Molecular Genetics
Via Selmi 3
Bologna (Italy)
tel. +0512094184
fax +0512094286

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