[Drosophila] mammalian promoters in insect cells
king-ferdi at gmx.net
Thu Mar 2 10:08:31 EST 2006
Dear fly experts,
I normally work with mammalian transformed cell lines (CHO, Cos, HeLa),
now I would like to do some experiments in drosophila cells (SL2,
KC167). Ideally I could transfect these cells with one of the constructs
I use for mammalian cells, but I am concerned if the promoters (SV40 and
CMV) will work in insect cells.
I searched the net on this problem and found no answer to this question.
Does anybody of you have experience with these promoters in the cell-
lines mentioned above or can refer to some info on the net?
Thanks in advance,
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