[Drosophila] Re: Dros Digest, Vol 11, Issue 2

[CRISTINA PALLARES CARTES]

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>    1. mammalian promoters in insect cells  (Fernando)
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> 
> Message: 1
> Date: Thu, 2 Mar 2006 16:08:31 +0100
> From: Fernando <king-ferdi at gmx.net>
> Subject: [Drosophila] mammalian promoters in insect cells 
> To: bionet-drosophila at moderators.isc.org
> Message-ID: <MPG.1e7128d83ae0f213989683 at news.mediascape.de>
> Content-Type: text/plain; charset="iso-8859-15"
> 
> Dear fly experts,
> 
> I normally work with mammalian transformed cell lines (CHO, Cos, HeLa), 
> now I would like to do some experiments in drosophila cells (SL2, 
> KC167). Ideally I could transfect these cells with one of the constructs 
> I use for mammalian cells, but I am concerned if the promoters (SV40 and 
> CMV) will work in insect cells.
> I searched the net on this problem and found no answer to this question. 
> 
> Does anybody of you have experience with these promoters in the cell-
> lines mentioned above or can refer to some info on the net?
> 
> Thanks in advance,
> 
> Fernando 


 
Hi Fernando.

It seems that the CMV promotor is not workin in S2 cells, but works fine in 
cl8+ cells.




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