[Drosophila] mites

_Elena via dros%40net.bio.net (by elena_lenalena from yahoo.com)
Mon Dec 3 12:39:30 EST 2007


Dr. Erickson lab protocol for get rid of mites (by dechorination):
 
1)      Let flies to lay eggs onto usual vial for overnight or 7 hours.
2)      Add  5 ml 50% bleach for 3 min
3)      pour out bleach (accurately). Check that eggs are on the surface of
the vial. 
4)      add 10 ml PBS 
5)      pour out PBS (accurately). Check that eggs are on the surface of the
vial
6)      add 10 ml PBS 
7)      pour out PBS (accurately). Check that eggs are on the surface of the
vial
8)      Let eggs to hatch 37oC 10 days.
 
Protocol#2 (less efficient)
 
1)      Transform flies every day onto new vial. Repeat 4 times. Grow flies
from the 4th vial.
2)       Repeat the same with the progeny from the 4th vial.


azza sellami wrote:
> 
> hello
> please can someone help me to irradicate mites
> thanks
> 

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