[Drosophila] BrDU Labelling

Christina Wagner via dros%40net.bio.net (by cwagner from zoologie.uni-kiel.de)
Mon Dec 17 11:41:48 EST 2007


Hallo everybody,
at the moment I'm trying to label tracheal cells in larvae (early  
first instar larvae) using a BrdU-antibody, by feeding the larvae a  
BrDu-fly medium mixture.
Unfortunately, I'm not successful, although this experiment is  
standardised in Drosophila labs worldwide. Can anybody do me a favour  
and send me a protocol (BrDu concentration, feeding time, andibody  
concentration!).
Thanks a lot
Christina 



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