[Drosophila] Curious Incident of the Flies in the Homozygote
(by soniasen from gmail.com)
Mon Dec 17 11:52:10 EST 2007
I was wondring if anyone could help me.
I have a stock which is such:
yhs FLP; GAD>GFP/CyOGFP; FRT82B tubGAL80/TM6B.
If I dissect out these flies which are balanced on both, I never see
GAD>GFP expression (as expected).
If I dissect out the flies that are homozygous on the third or
homozygous on both second and third I see that half of those brains
show the complete GAD>GFP expression.
The very same thing has happened to me with a very similar stock: yhs
FLP; GH146>GFP/CyOGFP; FRT82B tubGAL80/TM6B.
I dont think that it is because GAL80 is not enough because I see
complete scilencing in the balanced flies. Also because I have seen a
similar thing with two other stocks: w; GH146>2XeGFP and w;
In these stocks I never have a problem when the stock is kept
balanced, but as soon as I expand a homozygous copy of the stock into
bottles, I loose the GH146 expression.
I wanted to know if anyone has faced a similar problem and also if
anyone had any ideas as to why this was happening.
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