[Drosophila] RNA and RT-PCR

Dr. Arindam Basu via dros%40net.bio.net (by aribasu from vet.upenn.edu)
Wed Jul 11 19:47:25 EST 2007


Dear All,
   I am having problems with getting good cDNA synthesis with Drosophila total
RNA harvested from embryos. Quite a few times the actin PCR does not show a
good signal for checking the RT reaction. Are there any special step(s) for RNA
extraction and/or Reverse Transcription reaction (I use Invitrogen Superscript
kit for RT) to get optimum cDNA synthesis? 

Thanking you in advance
Arindam

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