[Drosophila] Larval Development

[Jack Gaynor]

Hi,

	I have been unable to locate the number of hours for the larval  
stages.  We have been setting up learning assays with 3rd instar  
larva and have been harvesting these at 100 hrs after egg laying.   
However, we are now trying to do the same for 1st and 2nd instar  
stages and are not sure exactly when to harvest them.  I realize this  
must be well know, but have not been able to locate the data.  We are  
culturing at 23C.

	Also, we have experimented with both Canton-S and Oregon R wild  
type.  I expected these to be very similar, but we have seen some  
significant differences in the response of 3rd instar larvae to light  
in these training assays.  Since we are relatively new to fly work,  
can anyone give us a sense of how similar or different these two wt  
strains are?  Which is the more "acceptable" line to work with?

	Many thanks!

Jack Gaynor


John J. Gaynor, Ph.D.
Department of Biology & Molecular Biology
Science Hall, SH114
Montclair State University
Montclair, NJ  07043

voice: 973-655-7253
fax: 973-655-7047
lab: 973-655-4075
email: jack.gaynor from montclair.edu