From chatterjee.n from gmail.com Thu Nov 1 15:08:40 2007 From: chatterjee.n from gmail.com (Nabanita Chatterjee) Date: Fri Nov 2 07:54:20 2007 Subject: [Drosophila] pTMR vector Message-ID: can someone please give me the sequence data for pTMR vector which is a descendant of pGMR ,the construct preparation was described in PLoS biology,jan 2004,Vol 2,Issue 1,Pg 0051 thanks nabanita From fu.qi.lai91 from gmail.com Fri Nov 2 21:51:59 2007 From: fu.qi.lai91 from gmail.com (fu. qi.lai) Date: Sat Nov 3 10:53:08 2007 Subject: [Drosophila] BMEI 2008, Sanya, China: Deadline 10 November In-Reply-To: <79afb2460711012222w3109d5ddkb1825315ac8916cb@mail.gmail.com> References: <79afb2460711012222w3109d5ddkb1825315ac8916cb@mail.gmail.com> Message-ID: <79afb2460711021951y3c1af01bh287ecc79a8dd22d3@mail.gmail.com> ** Our apologies if you receive multiple copies of this announcement * ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 2008 International Conference on BioMedical Engineering and Informatics (BMEI 2008) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 28 - 30 May 2008, Sanya, Hainan, China Submission Deadline: 10 November 2007 http://www.hainu.edu.cn/BMEI2008 Call for Papers, Invited Sessions & Sponsorship The aim of BMEI2008 is to bring together researchers working in many different areas of biomedical engineering and informatics to foster international collaborations and exchange of new ideas. The BMEI 2008 conference proceedings will be published by the IEEE and will be indexed in both EI and ISTP. Selected good papers will be recommended for publication in SCI/SCI-E indexed international journals. Keynote speakers include Professor Maciej Ogorzalek, President-Elect, IEEE Circuits and Systems Society, Editor-in-Chief, IEEE Circuits and Systems Magazine, IEEE Fellow; Dr. Roderic Pettigrew, Director of National Institute of Biomedical Imaging and Bioengineering (NIBIB), USA; Professor Jing Bai, IEEE Fellow. The BMEI 2008 will be co-located with the 2008 Congress on Image and Signal Processing (CISP 2008: http://www.hainu.edu.cn/CISP2008), in order to promote cross-fertilization between the broad areas of biomedical engineering and signal processing. ABOUT SANYA Sanya is one of China's premier tourist destinations, with white-sand beaches, charming scenery, hot-springs, and popular activities such as scuba-diving and rafting. More than 20 ethnic groups, including Han, Li, Miao, and Hui, inhabit Sanya and make Sanya a wonderful place to appreciate the various cultures of China. For more information, visit the conference web page or email the secretariat at bmei2008@hainu.edu.cn From imcdanie from ScrippsCollege.edu Fri Nov 9 20:15:15 2007 From: imcdanie from ScrippsCollege.edu (Ivy McDaniel) Date: Sat Nov 10 11:02:10 2007 Subject: [Drosophila] Getting rid of maternal contribution? Message-ID: <473495A30200009D00029610@SCRNCS1.scrippscollege.edu> Hello all, Here's my situation - I am trying to study the chromosomal mutant phenotype of a specific protein. I can make viable homozygous mutants, but they are sterile. Each time I want to do an experiment, I have to set up a cross using heterozygous parents. Here is my problem - there seems to be a great deal of maternal protein on the "mutant" chromosomes, that lasts until the end of the third-instar larval stage. I would like to do immunofluorescence on polytene chromosomes, so it would be lovely if there was a way to get rid of this maternal protein! The protein is frequently absent from the chromosomes if I pick extremely old larvae, but because they are so old they often appear to have defects that may not necessarily be mutant phenotypes. I have a construct for GAL4 driven RNAi knockdown, but I'm concerned that the maternal mRNA will be translated long before the onset of zygotic transcription. We don't have the capabilities to do injections. Does anyone have any ideas for reducing maternal contribution of a protein? And if not, does anyone know of a good driver that turns on almost immediately at the onset of zygotic transcription? Thank you all! Ivy From ccml06 from hndx02.hainu.edu.cn Sun Nov 11 09:56:35 2007 From: ccml06 from hndx02.hainu.edu.cn (CISP-BMEI2008) Date: Mon Nov 12 16:11:47 2007 Subject: [Drosophila] Extended Deadline: 1 December 2007, BMEI 2008, Sanya, China Message-ID: <200711112132003901874@hainu.edu.cn> ** Our apologies if you receive multiple copies of this announcement * ** As per author requests, we are extending the deadline for paper * ** submissions and invited session proposals to 1 December 2007. * ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 2008 International Conference on BioMedical Engineering and Informatics (BMEI 2008) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 28 - 30 May 2008, Sanya, Hainan, China Extended Submission Deadline: 1 December 2007 http://www.tjut.edu.cn/BMEI2008 Call for Papers, Invited Sessions & Sponsorship The aim of BMEI2008 is to bring together researchers working in many different areas of biomedical engineering and informatics to foster international collaborations and exchange of new ideas. The BMEI 2008 conference proceedings will be published by the IEEE and will be indexed in both EI and ISTP. Selected good papers will be recommended for publication in SCI/SCI-E indexed international journals. The BMEI 2008 will be co-located with the 2008 Congress on Image and Signal Processing (CISP 2008: http://www.tjut.edu.cn/CISP2008), in order to promote cross-fertilization between the broad areas of biomedical engineering and signal processing. ABOUT SANYA Sanya is one of China premier tourist destinations, with white-sand beaches, charming scenery, hot-springs, and popular activities such as scuba-diving and rafting. More than 20 ethnic groups, including Han, Li, Miao, and Hui, inhabit Sanya and make Sanya a wonderful place to appreciate the various cultures of China. For more information, visit the conference web page or email the secretariat at bmei2008@hainu.edu.cn 2007-11-11 CISP-BMEI2008 From ruben.cauchi from dpag.ox.ac.uk Thu Nov 15 13:35:32 2007 From: ruben.cauchi from dpag.ox.ac.uk (Ruben) Date: Fri Nov 16 08:17:03 2007 Subject: [Drosophila] In search of a low ubiquitous GAL4 driver on chromosome 2 Message-ID: <98C6629A-1A66-41F3-8949-B22F727F66A3@dpag.ox.ac.uk> Hello all, Does anyone know of a low ubiquitous (i.e. expressing in all tissues) GAL4 driver on chromosome 2? I am in search of a driver, the expression of which is far lower than the notorious Act5c-GAL4. I would appreciate any replies. Best wishes, Ruben From c.sinensis from gmail.com Fri Nov 16 10:12:19 2007 From: c.sinensis from gmail.com (Zhang Yi) Date: Sat Nov 17 09:49:45 2007 Subject: [Drosophila] What happened to Exelixis collection in Harvard? Message-ID: <200711162312139211196@gmail.com> 2007-11-16 Hi -- Just would like to know if anybody can proceed to order the Exelixis collection in HMS stock. The website (http://drosophila.med.harvard.edu/) seems to be permanantly in "upgrade" for a week or longer. Thanks a lot :) Best, Yi -- Zhang Yi Lab of Neuroscience, B109 National Institute of Biological Sciences, 7 Kexueyuan Rd. ZGC Life Sciences Park, Beijing 102206 China, People's Republic of http://algebra.yculblog.com From anuj.m.bhatt from googlemail.com Mon Nov 19 06:47:05 2007 From: anuj.m.bhatt from googlemail.com (Anuj Bhatt) Date: Mon Nov 19 09:20:48 2007 Subject: [Drosophila] gateway vector - pCASPER Message-ID: <29e97f150711190347j5c248db7l24b97e85e40b327b@mail.gmail.com> Hi, I'm trying to source a drosophila transformation vector (like pCASPER4, but) with attB1 and attB2 sites, to use to generate transgenic drosophila lines. I need a basic vector to transfer inserts from a pDONR clone. The plasmid would have the basic elements for transformation, selection of transgenics, a gateway cassette, and would have been tested for fly transformation. Is there such a (unsophisticated) vector available? I have visited the plasmid larder at flybase and am confused at the selection on offer, largely through my ignorance of fruit fly-omics (being a plant biologist). Any help will be appreciated. Anuj -- Anuj Bhatt Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB UK Tel: 00-44-1865-275815 Mobile: 07742880305 Fax: 00-44-1865-275074 e-mail: anuj.m.bhatt@gmail.com From km287 from cornell.edu Mon Nov 19 11:31:00 2007 From: km287 from cornell.edu (Katy Munson) Date: Mon Nov 19 11:54:18 2007 Subject: [Drosophila] gateway vector - pCASPER Message-ID: <4741BA44.8040409@cornell.edu> *I'm trying to source a drosophila transformation vector (like pCASPER4, but) with attB1 and attB2 sites, to use to generate transgenic drosophila lines.* Have you considered the Murphy Lab Gateway collection? http://www.ciwemb.edu/labs/murphy/Gateway%20vectors.html The most basic ones have either UASt or UASp promoters driving an untagged protein; you can also get versions with tags. They can be ordered from the DGRC once you've signed the correct MTAs. https://dgrc.cgb.indiana.edu/ -- Katy Munson Technician III Lis Lab Cornell University km287@cornell.edu From s.fahle from sheffield.ac.uk Wed Nov 21 10:22:23 2007 From: s.fahle from sheffield.ac.uk (SheffieldSarah) Date: Wed Nov 21 10:29:44 2007 Subject: [Drosophila] Wanted: Drosophila tolteca Message-ID: <13879248.post@talk.nabble.com> Hello, I'm wondering if anyone has any Drosophila tolteca flies? I need about 4 vials of (if possible) recently collected non-isofemale lines for an experiment I'm conducting. Please contact me at s.fahle@sheffield.ac.uk if you work with this species and could post me some. Cheers -- View this message in context: http://www.nabble.com/Wanted%3A-Drosophila-tolteca-tf4850801.html#a13879248 Sent from the Bio.net - Dros mailing list archive at Nabble.com. From zous from mail.nih.gov Sun Nov 25 23:16:58 2007 From: zous from mail.nih.gov (zous@mail.nih.gov) Date: Mon Nov 26 11:07:45 2007 Subject: [Drosophila] postdoc/biologist position Message-ID: <000001c82fe3$30959450$035cbb89@nia.nih.gov> A postdoctoral or biologist position is available in January 2008 to study aging in Drosophila melanogaster at the National Institute on Aging in Baltimore, Maryland. Candidates with strong background in fly genetics and molecular biology and interests in aging are encouraged to contact Dr. Sige Zou at zous@grc.nia.nih.gov. From ren2 from jhmi.edu Mon Nov 26 15:56:44 2007 From: ren2 from jhmi.edu (QIUTING REN) Date: Mon Nov 26 16:15:40 2007 Subject: [Drosophila] in situ mRNA hybridization on adult brain whole mounts Message-ID: Hi, does anyone have a detailed protocol for in situ mRNA hybridization on fly adult brain whole mounts? Thank you. Qiuting From dmerrill from ksu.edu Tue Nov 27 10:09:27 2007 From: dmerrill from ksu.edu (Doris Merrill) Date: Tue Nov 27 11:19:19 2007 Subject: [Drosophila] SYMPOSIUM - New Insights from Arthropod Genomes Message-ID: <002301c83107$82391410$78738281@ECOGENOFFICE> SYMPOSIUM: NEW INSIGHTS FROM ARTHROPOD GENOMES April 11 & 12, 2008, in Kansas City SYMPOSIUM PROGRAM: The main symposium sessions will take place on Friday-Saturday, April 11-12, with optional events on Thursday evening and Sunday morning. Speakers will present new insights from genomic approaches in arthropods and describe the development of tools for genomic analysis. FEATURED SPEAKERS: * John K. Colbourne, Indiana University * Christine G. Elsik, Texas A&M University * Sarjeet S. Gill, University of California * Catherine A. Hill, Purdue University * Thomas Kaufman, Indiana University * John Manak, NimbleGen * Hugh M. Robertson, University of Illinois at Urbana-Champaign * Bruce R. Schatz, University of Illinois at Urbana-Champaign * Jeff Stuart, Purdue University * Judith H. Willis, University of Georgia * Evgeny Zdobnov, University of Geneva Medical School POSTER SESSIONS: There will be two poster sessions. A few platform presentations will be chosen from submitted poster abstracts. WORKSHOPS AND ROUNDTABLE DISCUSSION: On Thursday evening, a workshop on community contributions to genome annotation will feature a presentation on use of the Apollo Genome Annotation Tool by Dr. Chris Elsik (BeeBase). Throughout the meeting, arthropod genome database and bioinformatics tool developers will be available for individual training. On Sunday morning, there will be a roundtable discussion led by members of the ArthropodBase Consortium regarding the generation of integrated arthropod genome databases and tools for genome projects. Symposium attendees are invited to participate in these additional events. VENUE: The symposium will take place at the historic Muehlebach Hotel (operated by Marriott) in downtown Kansas City. Participants are encouraged to stay Saturday night for an optional evening of jazz and KC barbeque. REGISTRATION: The registration fee will be $275 ($150 for graduate and undergraduate students), and will include a welcome reception Thursday evening, breakfast and lunch on Friday and Saturday, and light refreshments at the Friday poster session. Visit our website, www.k-state.edu/agc, for updates as details are finalized. Information regarding registration, poster submission, and hotel reservations will be posted in December. INFORMATION: Add your name to the Symposium mailing list, by sending your contact information to dmerrill@k-state.edu. Please share this announcement with colleagues and students! Susan J. Brown, Professor Director, Center for Genomic Studies on Arthropods Affecting Human, Animal and Plant Health by Doris Merrill, Program Coordinator K-State Arthropod Genomics Center Division of Biology, Kansas State University 116 Ackert Hall, Manhattan, KS 66506-4901 (785) 532-3482, dmerrill@k-state.edu www.k-state.edu/agc From wad23 from cam.ac.uk Tue Nov 27 12:44:51 2007 From: wad23 from cam.ac.uk (W.A. Doward) Date: Tue Nov 27 13:01:11 2007 Subject: [Drosophila] Cambridge University Survey Message-ID: 27 November 2007 Dear Flybase Member, My name is Dr William Doward and I am currently studying for an MBA at the University of Cambridge, UK. My study group and I are currently undertaking a consultancy project for Aktogen Limited, a Cambridge start-up company that provides bespoke genetic constructs and specialist laboratory equipment for the study of behaviour in the common fruit fly. Aktogen also specialises in performing fly behavioural studies relating to courtship, learning, memory, and locomotion (see company brief below). To better understand the above market, we are interested in contacting laboratories that are using the fruit fly model and are particularly keen to hear from researchers interested in fruit fly behaviour. In an attempt to gauge the state of the current market we would be exceedingly grateful if interested Flybase members could complete a short survey, linked below. The survey contains 9 questions in total and will not take you more than 3 minutes to complete. We know that your time is very valuable but we would greatly appreciate your help in trying to understand this specialist market better and also the needs of researchers in this field. We would also welcome any additional comments or feedback that you feel may be of use. Sincerely, Dr William Doward MBA 2007 Judge Business School University of Cambridge wad23@cam.ac.uk http://cambridge.qualtrics.com//SE?SID=SV_08QVowPrHKScJWQ&SVID=Prod Aktogen Limited is a Cambridge University start-up company founded in 2003 by Dr. Zoltan Asztalos, a researcher of fifteen years experience in fruit fly genetics, and is supported by a distinguished international Scientific Advisory Board. Aktogen offers a wide range of equipment and behavioural tests relating to the fruit fly, Drosophila melanogaster, although the company's behaviour equipment could equally be used in studies involving other insects. Its personnel have particular experience in designing and running behaviour tests involving fly courtship and locomotion. The company also offers services to generate transgenic Drosophila strains, including making custom designed genetic constructs and embryo transformation. For further information please visit Aktogen's website. http://www.aktogen.com/ From smm from email.arizona.edu Fri Nov 30 19:07:25 2007 From: smm from email.arizona.edu (Stacy Mazzalupo) Date: Sat Dec 1 12:04:14 2007 Subject: [Drosophila] Tucson Stock Center shipping Message-ID: <46871bd7c412b386753233ff641012dd@email.arizona.edu> Dear Drosophila researchers, The Tucson Stock Center will not be shipping orders during the holidays. Please feel free to order during this time, however due to the Post Office delivery schedule and holiday closures, we will not be shipping orders as follows: International no shipping after Dec. 11, 2007 until Jan. 2, 2008. US - only no shipping after Dec. 18, 2007 until Jan. 2, 2008. Thank you, Stacy Stacy Mazzalupo, PhD Assistant Staff Scientist Manager, Tucson Stock Center University of Arizona e-mail: smm@email.arizona.edu phone: (520) 626-6322 fax: (520) 626-5863