From serena.duchi2 from unibo.it Tue Apr 1 21:09:03 2008 From: serena.duchi2 from unibo.it (Serena Duchi) Date: Mon Apr 7 10:14:08 2008 Subject: [Drosophila] BrdU protocol Message-ID: Hi community! Does anyone could please send me the BEST protocol for BrdU staining on follicle cells clones in Drosophila egg chambers? Thanks in advance serena Serena Duchi Unit of Developmental genetics Lab of Molecular Genetics Via Selmi 3 Bologna (Italy) tel. +0512094184 fax +0512094286 From m.gopfert from uni-koeln.de Wed Apr 2 07:13:14 2008 From: m.gopfert from uni-koeln.de (Martin Gopfert) Date: Mon Apr 7 10:14:14 2008 Subject: [Drosophila] Postdoc position Message-ID: Job Posting Sensory Systems Lab, University of Cologne Post-doctoral position (BAT II a) to study Drosophila hearing This position, available immediately, offered for 1 year in the first instance, and potentially renewable for a further 4 years, is for a young post-doctoral scientist with experience in Drosophila genetics and an interest in sensory systems. The project will be devoted to the identification and functional characterization of genetic hearing defects. Applications should be addressed to Professor Dr. Martin Gopfert Sensory Systems Lab Institute of Zoology University of Cologne Weyertal 119 50923 Cologne, Germany E-mail: m.gopfert@uni-koeln.de -- From rachel from flymine.org Wed Apr 2 09:49:12 2008 From: rachel from flymine.org (Rachel Lyne) Date: Mon Apr 7 10:14:18 2008 Subject: [Drosophila] FlyMine version 12.0 released Message-ID: <47F39CE8.6050001@flymine.org> We have released version 12.0 of FlyMine. In this release we have added the genome annotation for ten additional /Drosophila/ species and homology data between the 12 /Drosophila/ species from the 12 genomes consortium (2007) paper (pubmed: 170994087). We have also added mRNA expression data from the BDGP in-situ project and genetic interactions for /D. melanogaster/, /C. elegans/ and /S. cerevisiae/ from the BioGrid database. In addition we have improved the style and layout of the list analysis page and added a number of new widgets for analysing lists of genes - for example, an orthologues widget (showing counts of orthologues in other organisms for a list of genes ) and a genetic interactions widget (showing genes that interact genetically with genes in a list). For a list of all changes see www.flymine.org/release-notes. For a full list of data sources see www.flymine.org/release-12.0/dataCategories.do For all types of feedback email support@flymine.org ********************************************************************** PLEASE TAKE OUR SURVEY - it should only take a couple of minutes and is accessible from the FlyMine home page. This will help us tailor FlyMine to our users and identify areas where you would like to see new or modified features. ********************************************************************** The FlyMine Team - About FlyMine - FlyMine is an integrated database of genomic, expression and protein data from Drosophila, Anopheles and C. elegans. As an integrated resource it is possible to run data mining queries that span domains of biological knowledge. FlyMine is a freely available resource funded by The Wellcome Trust. ----------------- From schmitzh from uni-bremen.de Tue Apr 8 03:48:29 2008 From: schmitzh from uni-bremen.de (Heike Schmitz) Date: Tue Apr 8 09:37:11 2008 Subject: [Drosophila] Transparent Drosophila Message-ID: <47FB315D.7000009@uni-bremen.de> Hello Drosophila Community, some days ago I found some flies in my isofemale-lines, which had a inflated and transparent abdomen. Does anybody knows this characteristics? Is this an infection? I am really afraid of loosing my iso-lines... Thank you! Heike Schmitz From donito from woh.rr.com Tue Apr 8 10:33:40 2008 From: donito from woh.rr.com (Don Shuff) Date: Tue Apr 8 11:40:43 2008 Subject: [Drosophila] Transparent Drosophila In-Reply-To: <47FB315D.7000009@uni-bremen.de> References: <47FB315D.7000009@uni-bremen.de> Message-ID: <20080408113340.aabd5bb1.donito@woh.rr.com> I've seen this before in a couple of my stocks as well. I would also like to know what causes this and if this is something to be concerned about. On Tue, 08 Apr 2008 10:48:29 +0200 Heike Schmitz wrote: > Hello Drosophila Community, > > some days ago I found some flies in my isofemale-lines, which had a inflated and transparent abdomen. Does anybody knows this characteristics? Is this an infection? I am really afraid of loosing my iso-lines... > > Thank you! > > Heike Schmitz > > From roshan.vijendravarma04 from imperial.ac.uk Tue Apr 8 13:14:55 2008 From: roshan.vijendravarma04 from imperial.ac.uk (Vijendravarma, Roshan K) Date: Tue Apr 8 13:47:55 2008 Subject: [Drosophila] RE: transparent Drosophila References: <200804081704.m38H43T18880@net.bio.net> Message-ID: <50B46338D54D214BAEAE2F0EC4C253A80320E1AE@icex1.ic.ac.uk> Dear both, Inflated and transparent abdomen can result due to heavy infection by microsporidia, I have come across these quite often while examining microsporidia infected D. melanogaster. There could however be other possible reasons I am unaware of. If it's a microsporidian infection then it is something to be concerned about. It can be easily verified by Giemsa staining the abdominal smears of suspected individuals and examining it under light microscopy. Hope this helps! Roshan From giebultj from science.oregonstate.edu Tue Apr 8 14:54:26 2008 From: giebultj from science.oregonstate.edu (Jaga Giebultowicz) Date: Wed Apr 9 08:01:35 2008 Subject: [Drosophila] Transparent Drosophila In-Reply-To: <20080408113340.aabd5bb1.donito@woh.rr.com> References: <47FB315D.7000009@uni-bremen.de> <20080408113340.aabd5bb1.donito@woh.rr.com> Message-ID: <002901c899b2$5a02f1f0$bfe1c180@science.oregonstate.edu> If you deal with flies that just emerged on CO2 plate, there is a time when their cuticle is not hardened yet and some of them seem to bloat like that. To test if this may be the reason, examine aged flies for this "phenotype" - there should not be any bloating among them. Jaga Giebultowicz Associate Professor Oregon State University Department of Zoology 3029 Cordley Hall Corvallis OR 97331 Ph: 541 737 5530 http://www.cgrb.oregonstate.edu/faculty/giebultowicz Never fall in love with your hypothesis. - Peter Medawar -----Original Message----- From: dros-bounces@oat.bio.indiana.edu [mailto:dros-bounces@oat.bio.indiana.edu] On Behalf Of Don Shuff Sent: Tuesday, April 08, 2008 8:34 AM To: Heike Schmitz Cc: dros@magpie.bio.indiana.edu Subject: Re: [Drosophila] Transparent Drosophila I've seen this before in a couple of my stocks as well. I would also like to know what causes this and if this is something to be concerned about. On Tue, 08 Apr 2008 10:48:29 +0200 Heike Schmitz wrote: > Hello Drosophila Community, > > some days ago I found some flies in my isofemale-lines, which had a inflated and transparent abdomen. Does anybody knows this characteristics? Is this an infection? I am really afraid of loosing my iso-lines... > > Thank you! > > Heike Schmitz > > _______________________________________________ Dros mailing list Dros@net.bio.net http://www.bio.net/biomail/listinfo/dros From handler from nersp.nerdc.ufl.edu Tue Apr 15 11:09:27 2008 From: handler from nersp.nerdc.ufl.edu (Al Handler) Date: Tue Apr 15 14:56:52 2008 Subject: [Drosophila] Asilomar Workshop registration Message-ID: Vth INTERNATIONAL WORKSHOP ON TRANSGENESIS AND GENOMICS OF INVERTEBRATE ORGANISMS Asilomar Conference Center, Pacific Grove, California, June 15th - 19th, 2008 Dear Colleagues, Thank you for your interest in attending the 5th International Workshop on Insect Transgenesis and Genomics to be held at Asilomar, Pacific Grove, California from June 15-19, 2008. The focus of the workshop is on the genomics and functional genomics of insects and other invertebrates, and the use of genetic transformation methods for genomics analysis and applications. The major topics to be discussed will be: 1. Genomics 2. Functional genomics 3. Transposition and recombination systems 4. Genetic tools (including analysis systems) and markers 5. Practical applications for transgenic strains 6. Risks and concerns of transgenic release Registration is now open at the following web site: https://webpay.ucr.edu/webpay/home Further information on the conference site can be found at: http://www.visitasilomar.com Submission of fees will be required by May 1st, but if this is a problem because of the short time-frame, please contact me regarding this and for further information on the workshop and abstract submission. For the organizing committee, Peter Atkinson peter.atkinson@ucr.edu 1-951-827-4792 (phone) 1-951-827-3086 (fax) Workshop Organizing Committee Peter Atkinson, UC, Riverside Sarjeet Gill, UC, Riverside Al Handler, USDA-ARS, Gainesville, Florida David O'Brochta, University of Maryland From kim from kimvdlinde.com Sun Apr 20 22:34:11 2008 From: kim from kimvdlinde.com (Kim van der Linde) Date: Mon Apr 21 08:08:03 2008 Subject: [Drosophila] Saving the name Drosophila melanogaster Message-ID: <480C0B33.3030309@kimvdlinde.com> Dear All, The genus Drosophila as currently defined is paraphyletic and to resolve this issue, sooner or later, the genus need to be split. Unfortunately, if the normal rules regarding naming of genera in this case is followed, Drosophila melanogaster would be renamed to Sophophora melanogaster as it is positioned within the subgenus Sophophora. In order to avoid wide-scale confusion and instability with regard to Drosophila melanogaster, a proposal (Case 3407) to conserve the name Drosophila melanogaster is currently under consideration with the International Commission on Zoological Nomenclature (http://www.iczn.org/). If approved, Drosophila melanogaster will become the type species for the genus Drosophila (currently Drosophila funebris). If this is not approved, Drosophila melanogaster will become Sophophora melanogaster as soon as the genus is split. For those interested in the case, or would like to find additional information, please visit http://www.kimvdlinde.com/professional/DrosophilaSplit.html were all relevant information is collected, included a reprint of the case in question (copied with explicit permission of the ICZN). The procedure at the ICZN is as follows: 1. A application (case) is made to the Commission with a specific request. (http://www.iczn.org/Cases.htm) 2. Interested people can send a comment to the commission regarding the case, for example to express their opposition to the case. (http://www.iczn.org/Comments_on_Cases.htm) 3. The commission votes on the case and publishes an opinion. (http://www.iczn.org/Opinions.htm) The case was published last December and the first comments have been published in the March issue (one in favour, two opposing), and more comments will follow in the next issues. I have no idea how long comments are accepted, but interested parties are invited to express their position regarding the case (http://www.iczn.org/Instructions_for_authors.htm). Regards, Kim van der Linde -- http://www.kimvdlinde.com From pswescott from ucdavis.edu Mon Apr 21 14:19:04 2008 From: pswescott from ucdavis.edu (Phyllis Wescott) Date: Tue Apr 22 11:12:42 2008 Subject: [Drosophila] Summary of replies about clever ideas for cooking fly food. Message-ID: <000001c8a3e4$907cd430$5042eda9@eve.ucdavis.edu> I was researching the Filamatic pump when I found your page. My name is Phyllis Wescott. I rund the Drosophila Facility at U.C.D. in Davis, CA. I would like to send you a recipe proven to be the best for feeding Drosophila. I prepare approximately 70 liters a day, but have prepared small amounts. I use a Brewer Automatic pipetter from Fisher. Check out their Drosophila site. I use a Dover Steam Kettle model #AE1-20. You can find any size, even a tabletop. But if you are cooking small amounts, Use a two burner stove, a small metal pot and a large metal spoon. Wooden spoons absorb moisture and can't be cleaned properly.- You have received much help and I hope I have given you something to think about. Phyllis Wescott From djclancy from gmail.com Thu Apr 24 07:21:42 2008 From: djclancy from gmail.com (djclancy@gmail.com) Date: Thu Apr 24 08:34:30 2008 Subject: [Drosophila] mitochondrial sequences available to phylogeneticist fro collaboration Message-ID: Hello I have the complete protein coding sequences of about 15 strains of melanogaster from around the world and would love to write them up in some way but 1/ I have no expertise and limited interest in phylogenetics and 2/ have no time. The sequences are all lovely and assembled in Bioedit by gene and by species and are all ready to go. I'm looking for a collaborator who is ready to take these, analyse them and write it up. One strategy is to get some measure of neutral nuclear variation, which probably exists in the literature, and compare the variations to that in D. sim. But that's just a suggestion. In any case it should be able to be a quick, relatively easy paper All approaches gratefully and carefully considered. And please supply the contact details of anyone else you think may be interested. Probably not students unless they are very experienced at paper writing. I will send a network tree and a table indicating amino acid differences upon request. Regards dave David J Clancy Research Fellow Department of Life Sciences The Open University Milton Keynes Buckinghamshire MK7 6AA UK Tel +44(0)1908-654812 Fax +44(0)1908-654167 From djclancy from gmail.com Thu Apr 24 07:27:35 2008 From: djclancy from gmail.com (djclancy@gmail.com) Date: Thu Apr 24 08:34:52 2008 Subject: [Drosophila] Re: high/low calorie fly food References: Message-ID: On 26 Feb, 13:23, Andre Faustino wrote: > Dear all, > > I am trying to conduct some tests and will need a recipe for high and low calorie fly food. > Can anyone provide me with these recipes (or let me know were I can find them)? > > Thank you so much for your help. > > Best regards, > > Andr? > _________________________________________________________________ > Need to know the score, the latest news, or you need your Hotmail?-get your "fix".http://www.msnmobilefix.com/Default.aspx look at the literature from Linda Partridge's lab.