[Drosophila] Re: Dros Digest, Vol 33, Issue 1

Arun Rajamohan via dros%40net.bio.net (by arajamoh from uwo.ca)
Sat Jan 5 12:56:04 EST 2008


Hi All,

Today I noticed that my previous mail contained only the first few  
words of what I intended to send. Sorry about that and here is the  
full version.

~
An interesting question. And I have also wondered about the same. My  
feeling is that some of the laboratories that attempted to reproduce  
the results published as early as 1990 did not continue their work due  
to a small learning curve associated with the technique. Also high  
variability in viability that will show up even with extremely minor  
change of conditions in the procedure - primarily embryonic stage  
selection for the procedure. I would be really interested in hearing  
from any other laboratories that have attempted cryopreserving D.  
melanogaster embryos and the various pitfalls that they encountered  
while trying to do it. For that matter I am also keen in knowing all  
the hearsay in this regard.

-Arun

On Jan 4, 2008, at 12:06 PM, dros-request from oat.bio.indiana.edu wrote:

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>   1. Preserving Drosophila by Freezing? (Qin, Hongtao)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Thu, 3 Jan 2008 16:30:48 -0500
> From: "Qin, Hongtao" <qinh from cshl.edu>
> Subject: [Drosophila] Preserving Drosophila by Freezing?
> To: <dros from magpie.bio.indiana.edu>
> Message-ID:
> 	<FC75C186F5F59B46901B9468326E25530204AD from mailbox10.cshl.edu>
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Greeting all,
>
> I noticed from the following link that detailed protocols for  
> preserving Drosophila by freezing were made available to the fly  
> community in 1993.
> http://www.bio.net/bionet/mm/dros/1993-April/000011.html
> However, it seems like preserving Drosophila by freezing is still  
> not an option for keeping fly stocks in many labs. Does anyone know  
> why? Is it because the protocols were not reproducible by other  
> labs, the cost was too high or other problems? Is any lab still  
> trying hard to develop a better protocol?
>
> Thanks,
> Hongtao
>
> Hongtao Qin
> Cold Spring Harbor Laboratory
> 1 Bungtown Road
> Cold Spring Harbor, NY 11724
> Phone: 516-367-6826
>
>
>
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> End of Dros Digest, Vol 33, Issue 1
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