[Drosophila] Dissecting drosophila fat bodies and nile red staining
mmspring from ncsu.edu
(by mmspring from ncsu.edu)
Mon Jan 14 22:27:41 EST 2008
I am wondering if anyone has an efficient method for dissecting and
isolating drosophila fat bodies from 3rd instar larvae so that they can
easily be stained. Also I am using Nile Red as the stain to visualize fat
bodies and was wondering if anyone else had used Nile Red with a regular
compound fluorescent microscope. Most of the literature I have found
describes using a confocal microscope and I am looking for the best
buffers to use and the right dilution of stain when making my slides.
Thanks for any help I can get.
Greg Gibson Laboratory
North Carolina State University
Raleigh, NC USA
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