[Drosophila] Drosophila embryo injection
(by sa08383 from wotan.mdacc.tmc.edu)
Fri Jan 25 12:28:42 EST 2008
Exactly which brand of tape are you using? I use Scotch double stick
style #MMM-665. If that's what you are using, then you may have to try
I dechorionate with bleach, wash with lots of H2O, some 0.2%
tritonX-100, and more H2O. I line the embryos up on a grape plate and
press the slide with the tape on top of them, hard enough for them to
stick, but not hard enough to smash them. I've had very good results
with this method.
On Jan 25, 2008, at 7:55 AM, Samar Ezzat wrote:
> Hallo everybody ,
> I tried to inject Drosophila embryos using double -sided tape , i
> can dechorionate embryos manually and row them on other double -sided
> tape but when i put 10 s oil even in very small amount embryos slip
> i will be very happy if i get any suggestion .
> Thanks a lot
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