[Drosophila] Re: Dros Digest, Vol 33, Issue 12

Gurudatta Baraka via dros%40net.bio.net (by jaiganesha from gmail.com)
Sat Jan 26 13:52:36 EST 2008


hi nice to see the details of the glue tape usally peopl use halocarobon 700
for the injections
do u use any specific oil  with the scorch tape ?
pelase suggest the product catalogue number etc
thanks '
datta.,


On Jan 26, 2008 12:03 PM, <dros-request from oat.bio.indiana.edu> wrote:

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>   1. Re: Drosophila embryo injection (Kathleen Gajewski)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Fri, 25 Jan 2008 11:28:42 -0600
> From: Kathleen Gajewski <sa08383 from wotan.mdacc.tmc.edu>
> Subject: Re: [Drosophila] Drosophila embryo injection
> To: Samar Ezzat <samarezzat0 from yahoo.com>
> Cc: dros from magpie.bio.indiana.edu
> Message-ID: <6cfdd4b1a711e29f97be21e4362125e6 from wotan.mdacc.tmc.edu>
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> Exactly which brand of tape are you using?  I use Scotch double stick
> style #MMM-665.  If that's what you are using, then you may have to try
> another oil.
>
> I dechorionate with bleach, wash with lots of H2O, some 0.2%
> tritonX-100, and more H2O.  I line the embryos up on a grape plate and
> press the slide with the tape on top of them, hard enough for them to
> stick, but not hard enough to smash them.  I've had very good results
> with this method.
>
> Good luck
>
> KG
>
>
>
>
>
> On Jan 25, 2008, at 7:55 AM, Samar Ezzat wrote:
>
> > Hallo everybody ,
> >
> >   I tried to inject Drosophila embryos using double -sided tape , i
> > can dechorionate embryos manually  and row them on other double -sided
> > tape  but when i put 10 s oil even in very small amount  embryos slip
> > .
> >
> >    i will be very happy if i get any suggestion .
> >
> >   Thanks  a lot
> >
> >
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> End of Dros Digest, Vol 33, Issue 12
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