[Drosophila] Protocols and methods to homogenize flies
(by andre_faustino from hotmail.com)
Thu May 29 07:24:58 EST 2008
I am trying to quantify proteins, sugars and lipids in flies but having tro=
uble with the homogenization step.
So far I am homogenizing flies with a motor pestle in PBS. Then I perform a=
series of spins to remove tissue.
I use the supernatant to conduct the tests, but I am having troubles in get=
ting sufficient amount of material, especially to conduct the tests to eval=
uate lipid content of my samples. This leads to problems in sample comparis=
on since different levels of homogenization lead to different results obtai=
Can someone please provide me with protocols and tips for homogenization of=
E-mail for the greater good. Join the i=92m Initiative from Microsoft.
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