[Drosophila] FlyAtlas.org: Major upgrade and 7 new tissues added
(by j.a.t.dow from bio.gla.ac.uk)
Wed May 20 18:02:17 EST 2009
http://flyatlas.org is an Affymetrix microarray-based atlas of gene
expression in multiple tissues of adult and larval Drosophila
melanogaster. It been accessed by over 12000 users in the last
sixmonths, from nearly everywhere except Alaska.
We are delighted to announce the addition of 7 new tissues!
adult fat body,
S2 cells (growth phase).
We have also updated our local copies of allied data (Biogrid,
Homophila, NetAffx etc) that are linked from FlyAtlas, and harmonised
what gets displayed for each gene across the different searches.
We take great pride in the purity of the dissections, as evidenced by
high enrichments relative to whole fly. Here are some interesting
selections you can check out on the database:
Of course, there will be some bleed-through to the head samples, but
novel gene CG13840, Ecdysone-inducible gene L1 and shaker cognate B are
all pretty specific.
adult fat body:
There's not much of this to be found in 7-day adults, so we see some
cross-talk with other tissues. You should use this data cautiously.
However, Turandot X (immunity) and and insulin receptor suggest that the
data are OK.
This is Venkat's trickiest dissection to date, and so perhaps it's not
surprising to see a bit of cross-talk with fatbody. But neuromancer1
(H15), muscle-specific-homeodomain-2 (tin) and Bteb2 ( a homologue of a
gene associated with Waardenburg syndrome) are good examples of specific
Warniu, doublesex-Mab related 99B and tailless are UTTERLY larval
CNS-specific (i.e. not in adult CNS either).
This is a pretty clean sample (though you might also expect some
bleed-through to adult or larval carcass). However, there are some
highly specific, under-researched genes, like CG34199, CG6696 and
CG13047, all at least 10x enriched. And ecdysis triggering hormone-2
(ETH) - WOW!
S2 cells (growth phase):
With an organism with such potent genetic tools, I confess to being
under-excited by cell lines. But there are some low-level transcripts we
haven't seen anywhere else, like Gustatory receptor 94a or the novel
Overall, then, these new tissues are useful both to illuminate the
tissues in question, and to add authority to the tissue specificity of
genes expressed elsewhere.
For scriptwriters, programmatic calls to the database are possible, e.g.
You can search with FBgn, CG number, Affy oligo, gene symbol or free text.
These data were prepared and placed in the public domain as a service to
the Drosophila community, by the UKs Biotechnology and Biological
Sciences Research Council (BBSRC) through their Investigating Gene
Function (IGF) and bioinformatics and biological resources (BBR)
initiatives. Over the last 4 years, our facility has provided 1000 free
Affymetrix arrays to the UK fly community. Further details at:
Julian Dow (j.a.t.dow from bio.gla.ac.uk)
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