[Drosophila] Re: Dros Digest, Vol 55, Issue 9
(by slawek from univ.rzeszow.pl)
Sun Nov 29 16:44:29 EST 2009
Please, check the page:
The system is described by worm people. I tried to set it up. My problem
was that all the materials were US based, it took ages to import them
through the ocean and some parts were missing. They used a LED lamp and
filters which are mounted inside the microscope. I finally got the
materials, but still had to change the current - an electrician made
something very simple. It didn't work for me, the bulb did not produce a
single wave length light. Maybe a simple extra filter would help or the
current was too weak. I admit I didn't have a good workshop who would help
me. You might try the same, it will be easier for you and not so expensive.
I would be interested to know if you get it working.
> Hello all in flyland and happy thanksgiving!
> TWO QUESTIONS:
> Question the first:
> I have a question about selecting GFP + and - embryos/larvae etc.
> I have access to a stereofluorescence scope, but wondered if there was
> a cheaper quicker way of finding GFP embryos without fighting for a
> few moments on the scope, using up the expensive Hg lamp etc.
> So i am in the midst of purchasing a gel documentation system, and
> note that some systems have the capacity to select GFP as well. Either
> through a filter on the camera or through a transluscent plate that
> you put on top of the transilluminator (that I guess has to be pretty
> broad spectrum - 302-560ish? to catch both uv for DNA work AND GFP
> My fantasy (OK I have no life) is to take my little egg collection
> plates with emrbyos on them and put them in the gel doc system and
> take a pic in bright field (i mean white light) and then GFP, then
> take the plate out and look at the pictures and pick off the relevant
> embryos. No waiting around while lamps heat up/explode, no fighting
> with other people for the one fluorescent scope etc.
> I use CyO-GFP Knirps, (don't know the stock#) and TM3-GFP BL6663.
> Don't have an X-linked one but if you know one that's good, be
> grateful for the info.
> Question the seconde.
> I heard a rumour that light sources for fluorescence microscopy are
> going LED soon. I mean there is that yucky bluish thing but if it
> works, it seems to me a major breakthrough (cheap, lasts forever etc).
> Any info? Any recs for scopes that use this? Companies etc? Please
> correct me if this is just something i made up and doesn't exist.
> Dept. Biology
> Western Washington University
> Bellingham WA
> Dros mailing list
> Dros from net.bio.net
> End of Dros Digest, Vol 55, Issue 9
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