[Drosophila] Re: Dros Digest, Vol 64, Issue 6-how to pick larvae

Atkins, Mardelle Renee via dros%40net.bio.net (by ma144323 from bcm.tmc.edu)
Sun Aug 22 16:17:35 EST 2010


Hi Li,

You can also pick wandering larvae effectively with a paintbrush or a dissecting probe.  You can also collect younger larvae by adding several mls of 20% sucrose to the vial or bottle.  The larvae will float and are easily pipetted off using a cut tip.  Be warned however, only do this on bottles/vials you are ready to discard.

Good luck.
Mardelle Atkins


On 8/21/10 12:05 PM, "dros-request from oat.bio.indiana.edu" <dros-request from oat.bio.indiana.edu> wrote:

Send Dros mailing list submissions to
        dros from net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
        http://www.bio.net/biomail/listinfo/dros
or, via email, send a message with subject or body 'help' to
        dros-request from net.bio.net

You can reach the person managing the list at
        dros-owner from net.bio.net

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Dros digest..."


Today's Topics:

   1. RE: how to collect Drosophila larvae (Venkateswara Chintapalli)


----------------------------------------------------------------------

Message: 1
Date: Fri, 20 Aug 2010 18:01:50 +0100
From: "Venkateswara Chintapalli" <v.chintapalli from bio.gla.ac.uk>
Subject: RE: [Drosophila] how to collect Drosophila larvae
To: "Li, Fei" <lifei03 from gmail.com>, <Dros from magpie.bio.indiana.edu>
Cc: Dros from magpie.bio.indiana.edu
Message-ID:
        <2B9DE69543FDE44FBD798FF119FD212F16764A from exchange-be6.centre.ad.gla.ac.uk>

Content-Type: text/plain;       charset="iso-8859-1"

Hi Li,

I collect wandering third instar larvae by picking them (as they wander around) from the food container wall with a blunt forceps . And feeding third instar (or other instars) larvae can be collected by placing the food (as they feed inside) in a petri dish and collect them on the basis of their size. Hope this helps.

Sincerely, Venkat
_________________________________________________________________
Venkateswara R Chintapalli
Institute of Molecular, Cell and Systems Biology,
School of Medical, Veternary and Life Sciences,
University of Glasgow, Glasgow, Scotland,
UK G12 8QQ
Tel 0141 330 6811
v.chintapalli from bio.gla.ac.uk

________________________________

From: dros-bounces from oat.bio.indiana.edu on behalf of Li, Fei
Sent: Fri 20/08/2010 09:08
To: Dros from magpie.bio.indiana.edu
Cc: Dros from magpie.bio.indiana.edu
Subject: [Drosophila] how to collect Drosophila larvae



Hi, Fruitfliers,

Does anyone have good ideas on collecting drosophila larvae?  Thanks a lot.

Fei
_______________________________________________
Dros mailing list
Dros from net.bio.net
http://www.bio.net/biomail/listinfo/dros




------------------------------

_______________________________________________
Dros mailing list
Dros from net.bio.net
http://www.bio.net/biomail/listinfo/dros

End of Dros Digest, Vol 64, Issue 6
***********************************



More information about the Dros mailing list