[Drosophila] Re: ubiquitous Gal4 driver without balancer

plamen georgiev (BI) via dros%40net.bio.net (by plamen.georgiev from bbsrc.ac.uk)
Mon Sep 6 08:44:58 EST 2010


I agree. I have used a da-Gal4 insertion on the 3rd that is a bit sickish but homozygous viable. It is not as strong as the Bloomington act-Gal4 or tub-Gal4 but stronger than arm-Gal4 and quite efficient in knocking down a gene of interest to cause up to 90-95% lethality...
________________________________________
From: dros-bounces from oat.bio.indiana.edu [dros-bounces from oat.bio.indiana.edu] On Behalf Of mahesh Reddy [maheshwar.gummalla from unige.ch]
Sent: 06 September 2010 10:58
To: bionet-drosophila from moderators.isc.org
Subject: [Drosophila] Re: ubiquitous Gal4 driver without balancer

have you tried using Daughterless driver line Da Gal4


Bakker.Hans from mh-hannover.de <Bakker.Hans from mh-hannover.de> wrote:

> I am currently using an Act5C-Gal4 driver for my RNAi screens. This one
> is balanced with CyO/GFP. I, however, need to analyse the flies in
> rather large batches for glycolipid composition and would like to avoid
> the sorting of larvea after the crossing. Is there any good ubiquitous
> Gal4 driver line that I can keep as homozygotes so all my flies will
> have the RNAi phenotype atter the crossing. The ones in the bloomington
> stocks all seems to require a balancer.
> Thanks for any help, Hans
>
> Dr. Hans Bakker
> Hannover Medical School
> Dept. Cellular Chemistry OE4330
> Build. I3, Level 4, Room 1291
> Carl-Neubergstrasse 1
> 30625 Hannover, Germany
> bakker.hans from mh-hannover.de
> +49.511.5328245
>
>
>


--
G.Maheshwar Reddy,
Graduate Student,
Dept.of Zoology and Animal Biology,
Sciences III
University of Geneva
30, quai Ernest-Ansermet
CH-1211 GENEVE-4

tel lab     ++41 22 379 6760
fax          ++41 22 379 6439

Maheshwar.Gummalla from unige.ch

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