[Drosophila] Re: Dros Digest, Vol 73, Issue 2

Catherine Eldred via dros%40net.bio.net (by eldrec from rpi.edu)
Sat May 14 22:28:58 EST 2011


Hi Julie-
It is possible to rig up your own CO2 apparatus with a side arm flask
attached by tubing to a flypad (available thru genesee scientific).  You ca=
n
disintegrate Alka Seltzer tablets in the flask.  The bubbles are CO2, and
will put your flies out for 5 or 10 minutes, more than long enough to sort
them under a dissection scope or with a hand-held magnifying glass.  When
they start to move around, just pop another tablet in the flask.  It might
be useful to find other solvents than water to dilute them in to maximize
your CO2 output - vinegar comes to mind, but may not be necessary.  I have =
a
friend who teaches high school biology, and this is the way he runs his fly
genetics unit.  Using this method shouldn't in any way damage the fertility
of your flies, and there are no flammable vapors to worry about.
Good luck,
Catherine Eldred

On Sat, May 14, 2011 at 1:03 PM, <dros-request from oat.bio.indiana.edu> wrote:

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> Today's Topics:
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>   1. Re: anesthetic of Drosophila (Julie Brill)
>   2. Re: Dros Digest, Vol 73, Issue 1 (Nam Chul Kim)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 13 May 2011 17:10:28 +0000
> From: Julie Brill <julie.brill from sickkids.ca>
> Subject: Re: [Drosophila] anesthetic of Drosophila
> To: Michael Ashburner <ma11 from gen.cam.ac.uk>, Maria Paula Zappia
>        <mpzappia from iibintech.com.ar>
> Cc: "dros from magpie.bio.indiana.edu" <dros from magpie.bio.indiana.edu>
> Message-ID: <C9F2DE41.A8F0%julie.brill from sickkids.ca>
> Content-Type: text/plain; charset=3D"Windows-1252"
>
> I=92ve been using triethylamine (aka FlyNap, according to the Carolina
> Biological Supply catalog) in my undergraduate third year fly lab (there
> were issues with us using ether in the lab and CO2 wasn=92t available).
>
> The main problem with triethylamine is that if the flies get wet (you hav=
e
> to stick a damp applicator into the vial around the plug), then they don=
=92t
> wake up and they don=92t reproduce (this is what happened the first year =
I ran
> the course and I had to rapidly come up with alternative labs for the
> students when I realized they wouldn=92t be looking at recombinant progen=
y
> from their crosses!).  This means that the applicator has to be thoroughl=
y
> drained so it doesn=92t drip on the flies (I have the TAs emphasize this =
every
> year).  Also, we anesthetize the flies in empty vials (no food) so they
> don=92t get stuck in the food.  It takes a while to knock them out and th=
ey do
> tend to keep twitching a bit even after they are anesthetized.  They star=
t
> to wake up about 50 minutes after being knocked out, so sometimes the
> students have to knock them out again if they are not done sorting or
> analyzing the flies.
>
> Overall, except for that first year, I have found that it works fine.
>  However, I personally hate the smell and am glad I am only in the lab ro=
om
> once a week.  I=92m curious about whether Michael thinks there=92s any re=
ason
> not to use triethylamine other than the smell.
>
> Best wishes,
> Julie Brill
>
>
>
>
> On 11-05-13 7:51 AM, "Michael Ashburner" <ma11 from gen.cam.ac.uk> wrote:
>
> No, use diethyl ether MA
>
> On 12 May 2011, at 22:57, Maria Paula Zappia wrote:
>
> > To whom it may concern
> > I am willing to set up a Drosophila Laboratory for my students in
> > order to
> > teach genetics. We do not have CO2 to use as anesthesic for the
> > fruitflies,
> > but I found that Fuyama (1977) proposed the use of triethylamine as an
> > anesthetic with prolonged effects. As we do have triethylamine in
> > the lab I
> > would like to know which dose of triethylamine is commonly used and
> > how to
> > use it.
> > I look forward to hearing from you
> > Best regards
> >
> > --
> >
> > Lic. Maria Paula Zappia
> > http://www.iib.unsam.edu.ar/
> > Instituto de Investigaciones Biotecnol=F3gicas
> > Universidad Nacional de General San Martin
> > IIB-INTECH UNSAM-CONICET
> > Edificio 24 y 19- Predio INTI
> > Colectora Av. Gral Paz 5445 e/ Albarellos y Constituyentes
> > Casilla de Correo 30
> > B1650KNA, San Martin
> > Prov. de Buenos Aires Argentina
> > Tel: (54 11) 4580-7255 al 57
> > Fax: (54 11) 4752-9639
> > _______________________________________________
> > Dros mailing list
> > Dros from net.bio.net
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>
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> ------------------------------
>
> Message: 2
> Date: Fri, 13 May 2011 13:58:21 -0500
> From: Nam Chul Kim <nck1992 from gmail.com>
> Subject: [Drosophila] Re: Dros Digest, Vol 73, Issue 1
> To: dros from oat.bio.indiana.edu
> Message-ID: <BANLkTimUGa613Tiq1oThshGavR97NLYKBw from mail.gmail.com>
> Content-Type: text/plain; charset=3DISO-8859-1
>
> Use Ice or ice packs.
> http://www.ableweb.org/volumes/vol-24/mini.5.ratterman.pdf
>
> On Fri, May 13, 2011 at 12:03 PM, <dros-request from oat.bio.indiana.edu>
> wrote:
>
> > Send Dros mailing list submissions to
> >        dros from net.bio.net
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> >        http://www.bio.net/biomail/listinfo/dros
> > or, via email, send a message with subject or body 'help' to
> >        dros-request from net.bio.net
> >
> > You can reach the person managing the list at
> >        dros-owner from net.bio.net
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Dros digest..."
> >
> >
> > Today's Topics:
> >
> >   1. anesthetic of Drosophila (Maria Paula Zappia)
> >   2. Re: anesthetic of Drosophila (Michael Ashburner)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Thu, 12 May 2011 18:57:12 -0300
> > From: Maria Paula Zappia <mpzappia from iibintech.com.ar>
> > Subject: [Drosophila] anesthetic of Drosophila
> > To: dros from magpie.bio.indiana.edu
> > Message-ID: <BANLkTinyfxdNvo1mj9+WFWgtf+kpbq8nzw from mail.gmail.com>
> > Content-Type: text/plain; charset=3DISO-8859-1
> >
> > To whom it may concern
> > I am willing to set up a Drosophila Laboratory for my students in order
> to
> > teach genetics. We do not have CO2 to use as anesthesic for the
> fruitflies,
> > but I found that Fuyama (1977) proposed the use of triethylamine as an
> > anesthetic with prolonged effects. As we do have triethylamine in the l=
ab
> I
> > would like to know which dose of triethylamine is commonly used and how
> to
> > use it.
> > I look forward to hearing from you
> > Best regards
> >
> > --
> >
> > Lic. Maria Paula Zappia
> > http://www.iib.unsam.edu.ar/
> > Instituto de Investigaciones Biotecnol=F3gicas
> > Universidad Nacional de General San Martin
> > IIB-INTECH UNSAM-CONICET
> > Edificio 24 y 19- Predio INTI
> > Colectora Av. Gral Paz 5445 e/ Albarellos y Constituyentes
> > Casilla de Correo 30
> > B1650KNA, San Martin
> > Prov. de Buenos Aires Argentina
> > Tel: (54 11) 4580-7255 al 57
> > Fax: (54 11) 4752-9639
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Fri, 13 May 2011 12:51:39 +0100
> > From: Michael Ashburner <ma11 from gen.cam.ac.uk>
> > Subject: Re: [Drosophila] anesthetic of Drosophila
> > To: Maria Paula Zappia <mpzappia from iibintech.com.ar>
> > Cc: dros from magpie.bio.indiana.edu
> > Message-ID: <43F055A1-D713-4539-92E4-4BAFD47D1D46 from gen.cam.ac.uk>
> > Content-Type: text/plain; charset=3DISO-8859-1; format=3Dflowed; delsp=
=3Dyes
> >
> > No, use diethyl ether MA
> >
> > On 12 May 2011, at 22:57, Maria Paula Zappia wrote:
> >
> > > To whom it may concern
> > > I am willing to set up a Drosophila Laboratory for my students in
> > > order to
> > > teach genetics. We do not have CO2 to use as anesthesic for the
> > > fruitflies,
> > > but I found that Fuyama (1977) proposed the use of triethylamine as a=
n
> > > anesthetic with prolonged effects. As we do have triethylamine in
> > > the lab I
> > > would like to know which dose of triethylamine is commonly used and
> > > how to
> > > use it.
> > > I look forward to hearing from you
> > > Best regards
> > >
> > > --
> > >
> > > Lic. Maria Paula Zappia
> > > http://www.iib.unsam.edu.ar/
> > > Instituto de Investigaciones Biotecnol=F3gicas
> > > Universidad Nacional de General San Martin
> > > IIB-INTECH UNSAM-CONICET
> > > Edificio 24 y 19- Predio INTI
> > > Colectora Av. Gral Paz 5445 e/ Albarellos y Constituyentes
> > > Casilla de Correo 30
> > > B1650KNA, San Martin
> > > Prov. de Buenos Aires Argentina
> > > Tel: (54 11) 4580-7255 al 57
> > > Fax: (54 11) 4752-9639
> > > _______________________________________________
> > > Dros mailing list
> > > Dros from net.bio.net
> > > http://www.bio.net/biomail/listinfo/dros
> >
> >
> >
> >
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> >
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> > End of Dros Digest, Vol 73, Issue 1
> > ***********************************
> >
>
>
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