[Drosophila] Re: Dros Digest, Vol 73, Issue 4

Nam Chul Kim via dros%40net.bio.net (by nck1992 from gmail.com)
Thu May 19 12:55:15 EST 2011


PLoS One.<http://www.ncbi.nlm.nih.gov/pubmed?term=CF2%20Represses%20Actin%2088F%20Gene%20Expression%20and%20Maintains>
2010
May 25;5(5):e10713.
CF2 represses Actin 88F gene expression and maintains filament balance
during indirect flight muscle development in Drosophila.
Gajewski KM<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Gajewski%20KM%22%5BAuthor%5D>
, Schulz RA<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Schulz%20RA%22%5BAuthor%5D>
.
Source

Department of Systems Biology, University of Texas M. D. Anderson Cancer
Center, Houston, Texas, USA. kmgajews from mdanderson.org

Find this paper. Flies described in this paper do not ubiquitously express
GFP or RFP but good for selecting green adults.
I have used some of these. they are really bright. you can get some from Dr.
Gajewski.


On Wed, May 18, 2011 at 12:03 PM, <dros-request from oat.bio.indiana.edu> wrote:

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>   1. other fluorescent colors of mel? (Susan Gershman)
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> Message: 1
> Date: Tue, 17 May 2011 12:38:22 -0700 (PDT)
> From: Susan Gershman <sgershm from gmail.com>
> Subject: [Drosophila] other fluorescent colors of mel?
> To: bionet-drosophila from moderators.isc.org
> Cc: howard.rundle from uottawa.ca
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> Hello,
> I am looking for mel stock in which a fluorescence protein is under
> the control of a ubiquitous promoter, not on a balancer, and is not a
> fusion protein (and ideally, without major fitness consequences). We
> need to be able to distinguish live adult marked flies from unmarked
> stock.
> We have dsred stock, and are looking for an additional color.
> We have been unsuccessfully attempting to use GFP stock. Because the
> marked animals glow in a wavelength similar to mel autofluorescence,
> we are having trouble distinguishing GFP flies from unmarked stock.
> Does anyone know of mel that fluoresce in any other colors?
> Thanks so much,
> Susan Gershman
>
>
>
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