[Drosophila] Imprecise or precise excision or deletion

[bharathi from ccmb.res.in]

(*= cross)
If the P-element is on X chromosome

Cross I        		Cross IA
P/P   *  FM3/FM7 	FM3/FM7   *    (transposase) delta 2-3 kinked sb
(red eye color)    				 


Cross II         
P/FM7 *   +/FM7 ; +/625   
(patchy red eye color)

Cross III   
    			P/FM7	   *FM7male
			(orange eye)

Cross IV  
  			P male  * FM3/FM7   (as many crosses as possible)
           		(white eye flies)

Cross V  		P/FM7  * P male (self crosses  from each vial)
			(white eye flies)

The homozygous lethal lines or homozygous viable with phenotype will be the p-element excision mutants


(*= cross)
If the P-element is on II chromosome

Cross I        		Cross IA
P/P   *  cyo/ tft 	cyo/tft   *    (transposase) delta 2-3 kinked sb
(red eye color)    				 


Cross II         
P/cyo       *      +/cyo ; +/625   
(patchy red eye color)

Cross III   
    			P/cyo  * cyo/tft
			(orange eye)

Cross IV  
  			P/cyo  * cyo/tft   (as many crosses as possible)

Cross V  		P/cyo  * P male (self crosses)
			(white eye flies)


The homozygous lethal lines or homozygous viable with phenotype will be the p-element excision mutants



(*= cross)
If the P-element is on III chromosome

Cross I        		Cross IA
P/P   *  TM3sb/TM6B 	TM3sb/TM6B *    (transposase) delta 2-3 kinked sb
(red eye color)    				 


Cross II         
P/Tb       *      625/Tb   
(patchy red eye color)

Cross III   
    				P/Tb  * TM3sb/TM6B
				(orange eye)

Cross IV  
  				P/Tb * TM3sb/TM6B (as many crosses as possible)

Cross V  		P/Tb  * P male (self crosses)
			(white eye flies)
The homozygous lethal lines or viable with phenotype will be the p-element excision mutants

 


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Sent: Wednesday, May 25, 2011 10:34:41 PM
Subject: Dros Digest, Vol 73, Issue 8

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Today's Topics:

   1. Imprecise or precise excision or deletion
      (Venkateswara Chintapalli)


----------------------------------------------------------------------

Message: 1
Date: Tue, 24 May 2011 16:28:04 +0100
From: "Venkateswara Chintapalli" <Venkat.Chintapalli from glasgow.ac.uk>
Subject: [Drosophila] Imprecise or precise excision or deletion
To: <dros from magpie.bio.indiana.edu>
Message-ID:
	<2B9DE69543FDE44FBD798FF119FD212F01F75F2C from exchange-be6.centre.ad.gla.ac.uk>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hi Drosophilists,
 
Could any of you guide me (schematic) to generate imprecise or precise excisions or deletions using p-element insertion lines available in the stock centres?
 
Any help would be highly appreciated.
 
Sincerely, Venkat
__________________________________________________________________________________
Venkat R Chintapalli
Institute of Molecular, Cell and Systems Biology,
School of Medical, Veternary and Life Sciences,
University of Glasgow, Glasgow, 
UK G12 8QQ
Tel 0141 330 6811
venkat.chintapalli from glasgow.ac.uk


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