[Drosophila] broad (Broad-Complex) stocks

Laurence Vonkalm via dros%40net.bio.net (by lvonkalm from ucf.edu)
Tue Jul 17 08:20:42 EST 2012


Dear Fly community members,

Our lab is reducing the number of broad (Broad-Complex) stocks we carry. Below are three lists of broad stocks we currently have in our lab.

All of the stocks on list 1 are available at the Bloomington stock center. This is not a complete list of broad stocks available at Bloomington.

List 2 are stocks not available at Bloomington that we will continue to maintain and send out to the fly community as requested.

List 3 are stocks that we intend to discard on August 1st. If you want any of these stocks please let us know before August 1st. We will ship the stocks by USPS unless you prefer FedEx and provide an account number.

Best wishes

Laurie

Laurie von Kalm
Department of Biology
University of Central Florida
Orlando, FL 32816-2368

lvonkalm from ucf.edu<mailto:lvonkalm from ucf.edu>
407-823-6684

List 1: Stocks we have that are also available at Bloomington. This is not a complete list of broad stocks at Bloomington.

Mutant alleles

br1
y1 br5 / Binsn
y1 br28 w1 /Binsn

y1 brrbp-1 / Binsn
y1 brrbp-4 / Binsn
y1 brrbp-5 / Binsn

y1 br2Bc-1 w1 / Binsn
y1 br2Bc-2 / Binsn
y1 br2Bc-4 / Binsn

y1 brnpr1-3 w1 / Binsn
y1 brnpr1-5  / Binsn
y1 brnpr1-6 / Binsn

y1 br2Bab-5 w1 / Binsn


In vitro constructs - broad cDNAs in casper-hs

527-5               brBRcore.Q1.Z1.hs.527-5                    chromosome 2
708-14             brBRcore.TNT.Q1.Z1.hs.708-14          chromosome 2
cD5-1              brBRcore.Z2.hs.cD5-1                           chromosome 3
797-3               brBRcore.Z3.hs.797-3                           chromosome 2
Z4-11               brBRcore.Z4.hs.Z4-11                            chromosome 3

List 2: Stocks not available at Bloomington that we will continue to maintain.

Mutants

br28 / Binsn                                                                                                (spontaneous P insertion)
br28 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f                        Dp(1:Y)y2 67g19.1 = Dp(1:Y)1A;2B17-18

y1 brrbp-1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f
y1 brrbp-2 w1/ Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 br2Bc-1 w1 / FM7 / Dp(1:Y)y2 67g19.1

y1 brnpr1-1 w1 sn1 / Binsn
y1 brnpr1-4 w1 / Binsn
T(1:3) brnpr1-7 y1 w1 / Binsn             (T(1;3)2B5; 61F3)

l(1) lpr-2 y1 w1 / Binsn                    (broad+ lethal from Kiss 1978 EMS screen = nprl3 sibling)
l(1) dlg-1 y1 w1 sn3 / Binsn            (broad+ lethal from Stewart 1972 EMS screen = nprl1 sibling)

Df(1)S39, cho2/ FM6l / Dp(1:Y)y2 67g19.1               (Df(1)1E1-2; 2B5-6)

y1 l(1) t242 / Dp(1:Y) Sz280 / C(1)DX1, y f
                                (Dp(1:Y)Sz280 = Dp(1:Y)1A; 2C1-2 with internal deficiency 2B3-4; 2B7-8)


In vitro constructs - broad cDNAs in casper-hs

527-14             brBRcore.Q1.Z1.hs.527-14                 chromosome 2

708-4               brBRcore.TNT.Q1.Z1.hs.708-4              chromosome X
708-1               brBRcore.TNT.Q1.Z1.hs.708-1              chromosome 3

cD5-4C           brBRcore.Z2.hs.cD5-4C                      chromosome 2
cD5-73H         brBRcore.Z2.hs.cD5-73H                    chromosome X

797-E8            brBRcore.Z3.hs.797-E8                      chromosome 3

Z4-6                brBRcore.Z4.hs.Z4-6                              chromosome X


In vitro construct - Manduca sexta broad Z4 cDNA in casper-hs

MsZ4-1A        (chromosome 3)                    Bayer et al. (2003) Dev Genes Evol 213, 471.

MsZ4-34; MsZ4-1A (chromosome 2 and chromosome 3)




List 3: broad stocks we will discard on August 1st.

Mutants

In(1) br6, y1 / Binsn      (In(1)2B3-4; 3C1)
y1 br8 / Binsn
y1 br27 w1
Df(1) br26, y1 w1 / Binsn       (Df(1)1D-E; 2B5)
y1 br8 / FM6 / Dp(1:Y) dor YT3
Df(1) br26, y1 w1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f
In(1) br6, y1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 brrbp-5 wa sn3 / Binsn
y1 brrbp-4 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 br2Bc-1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 brnpr1-6 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f
T(1:3) brnpr1-7 y1 w1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 br2Bab-1 / Binsn
y1 br2Bab-3 / Binsn
y1 br2Bab-1 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 br2Bab-3 / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f



Df(1)S39, wa spl v f car / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f        (Df(1)1E1-2; 2B5-6)
Df(1)S39, cho2  / Dp(1:Y)y2 67g19.1 / C(1)DX1, y f

y1 l(1) t242 / Binsn     (broad+ lethal mutation from 1982 Belyaeva EMS screen)

y1 dort257 / Binsn      (dor- allele from 1982 Belyaeva EMS screen)



In vitro constructs - broad cDNAs in casper-hs

527-5; 708-1               brBRcore.Q1.Z1.hs.527-5; brBRcore.TNT.Q1.Z1.hs.708-1
cD5-4C; cD5-1           brBRcore.Z2.hs.cD5-4C; brBRcore.Z2.hs.cD5-1
797-3; 797-E8             brBRcore.Z3.hs.797-3; brBRcore.Z3.hs.797-E8
Z4-13; Z4-11               brBRcore.Z4.hs.Z4-13; brBRcore.Z4.hs.Z4-11


In vitro construct - Salivary gland-specific gene L71-6 reporter

L71-6:LacZ                 (chromosome 2)                                Crossgrove et al. (1996) Dev Biol 180, 745.



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