[Drosophila] Re: EMS mutation recovery

dr julien colomb via dros%40net.bio.net (by julien.colomb from gmx.de)
Fri Nov 23 10:12:37 EST 2012


If you used two bottles for your stocks and get them separated, you may 
try to cross them together, mixing modifiers and getting the initial 
phenotype back. I have heard it works for mbm mutant, but of course, 
your situation is different.
> Dear Amira,
>
> Write something more, what the homozygous phenotype is.  Are your
> mutants alleles of one gene? Your second chromosome caught lethal
> mutation(s) and you can get rid of them, but to choose the best strategy
> we need more data.  Another point, which might be important: what
> markers are present on the chromosome(s).
>
> Best regards,
> Slawek.
>
> W dniu 2012-11-21 18:03, dros-request from oat.bio.indiana.edu pisze:
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>>     1. EMS mutation recovery (Amira Menfis)
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>> ----------------------------------------------------------------------
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>> Message: 1
>> Date: Tue, 20 Nov 2012 10:34:59 -0500
>> From: Amira Menfis <amiramenfis from gmail.com>
>> Subject: [Drosophila] EMS mutation recovery
>> To: dros from magpie.bio.indiana.edu
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>> Dear All,
>>
>> I have couples of EMS flies kept with balancer over many years. At the
>> beginning, all of them give homozygous, but now none of them give
>> homozygous offsprings. I do not know the exact localization of the EMS
>> mutations in chromosome. I only know they are located on 2nd
>> chromosome. Is
>> there anyway I can recover the flies to give homozygous? Thank you so
>> much!
>>
>> best
>>
>> amira
>>
>>
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