GFP toxicity (in E. coli?)

Bob Kodrzycki bkodrzyc at AWOD.COM
Wed Nov 29 13:04:39 EST 1995


We are having a problem with subcloning the modified GFP (by Haseloff) to
include
a strong promoter.  It may be that we are having general cloning problems
but now I am 
suspecting toxicity in the E.coli.  Does anyone have suggestions as to
E.coli strains that work well, or possibly cloning vectors?  Currently we
are using pUC 19 or pBluescript as a vector
and DH5a, HB101, or XL-2 E.coli.

Any input would be appreciated.

Thanx...Bob K
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  Bob Kodrzycki                 bkodrzyc at awod.com
  Westvaco Forest Research      (803) 851-4775
  Summerville, SC 29484         (803) 875-7185 FAX

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