GFP toxicity (in E. coli?)

Jack T Trevors jtrevors at uoguelph.ca
Wed Nov 29 16:38:55 EST 1995


Do you have any specific ideas on the nature of the toxicity and if it is 
related to plasmid copy number.
Professor Jack Trevors
University of Guelph
Guelph, Ontario
Canada N1G 2W1

On 29 Nov 1995, Bob Kodrzycki wrote:

> We are having a problem with subcloning the modified GFP (by Haseloff) to
> include
> a strong promoter.  It may be that we are having general cloning problems
> but now I am 
> suspecting toxicity in the E.coli.  Does anyone have suggestions as to
> E.coli strains that work well, or possibly cloning vectors?  Currently we
> are using pUC 19 or pBluescript as a vector
> and DH5a, HB101, or XL-2 E.coli.
> 
> Any input would be appreciated.
> 
> Thanx...Bob K
> ----------------------------------------------------
> ----------------------------------------------------
> 
>   Bob Kodrzycki                 bkodrzyc at awod.com
>   Westvaco Forest Research      (803) 851-4775
>   Summerville, SC 29484         (803) 875-7185 FAX
> 
> ----------------------------------------------------
> ----------------------------------------------------
> 
> 
> 



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