Help with finding a suitable scanner

Graham Davies gdavies at lshtm.ac.uk
Mon Sep 18 14:07:46 EST 1995


Hello.
I am posting this for a friend who has the following problem.

: 	Texas red is a fluorescent dye whose excitation wavelength is 589 nm.
: This is bound to albumin and then put into serum.  The resulting cocktail is 
: then eletrophoresed to separate the plasma proteins from the indigenous
: albumin as well as the texas red labeled albumin.  By using fluorescence I
: would like to measure the quantity of labeled albumin present.
:	Is there a scanner that can read the whole gel and present the data to
: a PC on which I am running NIH Image or Image Grabber.
:	If I can use a fluorescent scanner this will rapidly help to determine 
: the quantity of texas red present.  One consideration to think about is that 
: albumin by itself will fluoresce but this is less than 589nm - the upper 
: fluorescent reach of the serum proteins is about 450 nm.
:
: Thank you for helping
:
: Ian

Please reply to either  gdavies at lshtm.ac.uk  or  i.maconochie at ic.ac.uk

Many thanks

Graham.



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