GFP in animal virus

vcore vcore at COLLEGE.MED.UFL.EDU
Thu Aug 1 13:10:19 EST 1996


> rec. GFP-virus
> 
> (WMULL at ms-azm-3.azm.nl)
> 31 Jul 1996 02:42:58 -0700
> 
>    *  Messages sorted by: [ date ][ thread ][ subject ][ author ]
>    *  Previous message: Dr Andrew J. Doherty: "Re: GFP expression in
>      CHO"
> 
> --------------------------------------------------------------------
> 
> To: fluorpro at net.bio.net
> 
> From: WMULL at ms-azm-3.azm.nl ("LMIB   MULLERS  WIL")
> 
> Subject: rec. GFP-virus
> 
> Date: 31 Jul 1996 02:42:58 -0700
> 
> Question,
> 
> Who has experience with recombinant animal virus expressing GFP, to
> be able to localize the virus in organ sections, as an alternative
> for Lac-Z ?. Until now I haven't found articles dealing with this
> matter, except for a plant virus (Plant-J. 7(6), 1995). Are low
> amounts of virus in a cell really critical for detection of GFP
> expression?. Can this significantly be improved by: a strong
> promoter,enhancer region/ GFP mutants yielding greater brightness/
> localization of GFP to subcellular compartments ??. Maybe I missed a
> 
> previous discussion on this subject, being just recently introduced
> to this newsgroup. Experiences and suggestions are very welcome !
> 
> Rien Blok
> --------------------------------------------------------------------
> 
>    *  Previous message: Dr Andrew J. Doherty: "Re: GFP expression in
>      CHO"




Please check J. of Virology, 1996, v.70, #7, p.4646-4654.

Hope it helps. 

Regards, 
Sergei.





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