Relative fluorescence of GFP / molar fluorescence yield

Sam Witherspoon sw11527 at usav01.glaxo.com
Tue Dec 3 20:00:37 EST 1996


frank parlati wrote:
> 
> Does anyone know how many GFP molecules per unit volume (in particular the
> S65T and Y66H/Y145F mutants) are required to observe a signal by
> microscopy.

Along the same lines,  does anyone have any information on molar 
fluorescence yield for GFP of GFP S65T?  How does it compare to 
fluorescein on a per molecule basis?

Thanks
------------------------------------------------------------------------Sam Witherspoon                             e-mail:     sw11527 at glaxo.com
Flow Cytometry and Cell Sorting Resource    Tel:        919-483-3078
Dept. of Receptor Biochemistry              FAX:        919-483-3777
Glaxo Wellcome R&D
5 Moore Dr.      RTP, NC,  27709 USA



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