autofluorescence

jay brewster jay at MRI8.MRI.MONTANA.EDU
Tue Dec 3 17:44:10 EST 1996


Newsnet users;
I have a reporter mouse line expressing EGFP to characterize a promoter.  I am getting 
good signal in subsets of cells in several tissues.  I am using a HiQ FITC cube and am 
getting quite a bit of autofluorescence in some tissue.  What is the best way to deal 
with this? (another filter?, an addition step to eliminate the autofluorescent?).  
Also what is the best way to fix and mount tissue for GFP, I seem to be getting 
photobleaching in Fluorosave.  Thanks!   jay at po.mri.montana.edu



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