Drosophila cuticle autofluorescence

John Rebers jrebers at nmu.edu
Mon Dec 16 18:52:48 EST 1996


I'm interested in using GFP as a reporter to study expression of a Manduca
cuticle gene, by transfecting Drosophila with a Manduca cuticle promoter/GFP
fusion, and then examining expression of the GFP in epidermal tissues.  My
concern is that Drosophila cuticle has a yellow-green autofluorescence.  How
hard is this to distinguish from GFP fluorescence?  Would something like one
of the blue-fluorescent proteins work, or would it best to use
beta-galactosidase or another gene as the reporter?

Thanks for any advice.
John Rebers
Department of Biology
1401 Presque Isle Avenues
Northern Michigan University
Marquette, MI  49855
906-227-1585 (office)
906-228-3617 (home)
906-227-2013 (FAX)
e-mail address: jrebers at nmu.edu
Note: do not send mail to jrebers at pop.mail.nmu.edu or to jrebers at VM.NMU.EDU
- these may appear in the header, but will not work for my address.  Thanks!




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