(no subject)

Brandt Cassidy bgcassidy at noble.org
Tue Jan 2 18:34:24 EST 1996


In article <4c1fv5$jrm at itssrv1.ucsf.edu>, Brian Wickes
<wickes at cgl.ucsf.edu> wrote:

> Has anyone had difficulty expressing GFP-1 in bluscript?  How did you 
> make your construct (using SK+ and GFP from pGFP-1) and was a longwave 
> hand held UV light ok?
> 
> Brian

We found that only some of the colonies from the transformation gave very
high fluorescence. We did use a hand held UV light source to detect the
Fluorescence.  It helps to have a dark room to see it. A nontransformed E.
coli control is good for comparison as well.

Brandt G. Cassidy
Assistant Scientist
Plant Biology
The Samuel Roberts Noble Foundation
e-mail  BGCASSIDY@ noble.org



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