quantitation of GFP

Brandt Cassidy bgcassidy at noble.org
Wed Jul 3 18:28:22 EST 1996


I was wondering if anyone has developed a quantitative assay for GFP using
a fluorimeter? We are bombarding plant tissues and electroporating
protoplasts and would like to quantitate the level of GFP expression. We
thought that some form of cell lysis and fluorimitry might be a way to
quantitate the amount of GFP. Any ideas or other help with this would be
appreciated.

Brandt G. Cassidy
Assistant Staff Scientist
Plant Biology
The Samuel Roberts Noble Foundation
e-mail  BGCASSIDY at noble.org



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