Restriction sites in GFP. look here for hGFP.

INFMEDLAB mamocell at INET.UNI-C.DK
Tue Jul 16 07:23:41 EST 1996


This is the restrictionmap of hGFP from phGFP (phGFP, CVU43284). hGFP:
826-1545 bp.

This is a search for many restriction sites using the NTI vector 3.0
program.
					Gregers J. Gram, july 1996.



                                        ScrFI                    
                                        ~~~~~~                   
                                        NciI                TaqI 
                                        ~~~~~~                 ~ 
                                         MslI             ScrFI  
                                      ~~~~~~~~~~~         ~~~~~  
          NlaIII                        HpaII    NlaIV   EcoRII  
          ~~~                           ~~~~~   ~~~~~~~   ~~~~~  
          MslI                          DsaV      BmyI    DsaV   
          ~~~~~~                        ~~~~~~   ~~~~~~~  ~~~~~  
            HphI              AluI       BsaJI   BanI     BstNI  
            ~~~~~             ~~~~~     ~~~~~~~ ~~~~~~~   ~~~~~  
     826  ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 
          TACCACTCGT TCCCGCTCCT CGACAAGTGG CCCCACCACG GGTAGGACCA 
                          MnlI       HphI              FokI      
                          ~~~~       ~~~~~             ~~~~~     

          TaqI                   HaeIII                HpaII     
          ~~~                     ~~~~                 ~~~~      
            AluI         MaeII    EaeI             BspWI         
            ~~~~         ~~~~    ~~~~~~         ~~~~~~~~~~~~     
     876  CGAGCTGGAC GGCGACGTGA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG 
          GCTCGACCTG CCGCTGCACT TGCCGGTGTT CAAGTCGCAC AGGCCGCTCC 
                                                            MnlI 
                                                            ~~~~ 

               BcgI                 AluI     Eco57I        BspWI 
           ~~~~~~~~~~~~~            ~~~~     ~~~~~~        ~~~~~ 
     926  GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC 
          CGCTCCCGCT ACGGTGGATG CCGTTCGACT GGGACTTCAA GTAGACGTGG 
            MnlI  SfaNI                                   BsgI   
            ~~~~  ~~~~~~                                 ~~~~~~  

                              ScrFI                              
                              ~~~~~~                             
                                 Sau96I                          
                                  ~~~~~                          
                             EcoRII                              
                              ~~~~~~                             
                              DsaV                               
                              ~~~~~~                             
                      BspWI   BstNI                              
                  ~~~~~~~~~~~~~~~~~~                             
           HpaII           BsiYI         MslI                    
           ~~~~         ~~~~~~~~~~~~  ~~~~~~~~~~~                
           BsrFI Fnu4HI       BsaJI      MnlI                    
          ~~~~~~  ~~~~~~     ~~~~~~~     ~~~~~                   
          BspWI  AluI      BmyI  HaeIII      MaeIII              
          ~~~~~~ ~~~~~    ~~~~~~~ ~~~~        ~~~~~              
     976  ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCACCTA 
          TGGCCGTTCG ACGGGCACGG GACCGGGTGG GAGCACTGGT GGAAGTGGAT 
                  BbvI                                   HphI    
                  ~~~~~~                                 ~~~~~   

                         BspWI                                   
                      ~~~~~~~~~~~~                               
               BspWI       Fnu4HI                  Fnu4HI        
            ~~~~~~~~~~~~    ~~~~~~                  ~~~~~~       
               BsgI          AciI            NlaIII BbvI         
              ~~~~~~         ~~~~~            ~~~~  ~~~~~~       
    1026  CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT 
          GCCGCACGTC ACGAAGTCGG CGATGGGGCT GGTGTACTTC GTCGTGCTGA 
                       Eco57I                                    
                       ~~~~~~                                    

                                            ScrFI                
                                            ~~~~~                
                                       MaeII                     
                                       ~~~~~                     
                                           EcoRII                
                                            ~~~~~                
                       NlaIII               DsaV HinP1I          
                        ~~~~                ~~~~~ ~~~~~          
                   AciI                     BstNI CfoI           
                   ~~~~~                    ~~~~~ ~~~~~          
    1076  TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 
          AGAAGTTCAG GCGGTACGGG CTTCCGATGC AGGTCCTCGC GTGGTAGAAG 
                                                           MboII 
                                                              ~~ 
          MboII                                            MboII 
          ~~~~~                                            ~~~~~ 

                                       HinP1I                    
                                        ~~~~~                    
                                        CfoI                     
                                        ~~~~~                    
                                       BstUI    HphI             
                                       ~~~~~    ~~~~~            
                                         BsiYI                   
                                      ~~~~~~~~~~~~               
                                      AciI   BsaJI       TaqI    
                                      ~~~~  ~~~~~~       ~~~~    
    1126  TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 
          AAGTTCCTGC TGCCGTTGAT GTTCTGGGCG CGGCTCCACT TCAAGCTCCC 
          MboII                               MnlI         MnlI  
          ~~~                                 ~~~~         ~~~~  

                ScrFI                                            
                ~~~~~~                                           
                MslI                                             
             ~~~~~~~~~~~                                         
                   HphI                                          
                   ~~~~~~                                        
               EcoRII                                            
                ~~~~~~                                           
                DsaV            TaqI                             
                ~~~~~~          ~~~~                             
                BstNI       SfaNI    Eco57I     TaqI             
                ~~~~~~      ~~~~~~   ~~~~~~~    ~~~~             
                BsaJI     AciI     AluI      SfaNI               
               ~~~~~~~    ~~~~     ~~~~      ~~~~~               
    1176  CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG 
          GCTGTGGGAC CACTTGGCGT AGCTCGACTT CCCGTAGCTG AAGTTCCTCC 
                                                            MnlI 
                                                            ~~~~ 

                     ScrFI                                       
                     ~~~~~                                       
                   EcoRII                                        
                     ~~~~~                                       
                     DsaV                                        
                     ~~~~~                                       
                     BstNI              RsaI                     
                     ~~~~~              ~~~~~                    
                      BsaJI         BpmI                         
                     ~~~~~~        ~~~~~~                        
                          BmyI   AluI   Csp6I              MaeII 
                         ~~~~~~  ~~~~   ~~~~~                 ~~ 
    1226  ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC 
          TGCCGTTGTA GGACCCCGTG TTCGACCTCA TGTTGATGTT GTCGGTGTTG 
                 FokI                                            
                 ~~~~~~                                          

                 NlaIII                                          
                  ~~~~~                                          
                     HaeIII                     MslI             
                      ~~~~                   ~~~~~~~~~~~         
          MaeII       EaeI         MboII    SfaNI  HphI    BstYI 
          ~~         ~~~~~~        ~~~~~    ~~~~~  ~~~~~~      ~ 
    1276  GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 
          CAGATATAGT ACCGGCTGTT CGTCTTCTTG CCGTAGTTCC ACTTGAAGTT 

          DpnII                          Fnu4HI                  
          ~~~~                             ~~~~~                 
          DpnI                           BsgI                    
          ~~~~                          ~~~~~~~                  
          BstYI                   Fnu4HI   BbvI                  
          ~~~~~                    ~~~~~   ~~~~~                 
             AciI        TaqI      BbvI      AluI                
             ~~~~        ~~~~      ~~~~~     ~~~~                
    1326  GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC 
          CTAGGCGGTG TTGTAGCTCC TGCCGTCGCA CGTCGAGCGG CTGGTGATGG 
          AlwI             MnlI                                  
          ~~~~~            ~~~~                                  

                                  Sau96I                         
                                   ~~~~~                         
                                    NlaIV     Fnu4HI             
                                   ~~~~~~      ~~~~~             
                                  HaeIII   Fnu4HI                
                                   ~~~~     ~~~~~                
    1376  AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC 
          TCGTCTTGTG GGGGTAGCCG CTGCCGGGGC ACGACGACGG GCTGTTGGTG 
                                               BbvI              
                                               ~~~~~             
                                            BbvI                 
                                            ~~~~~                

                                                         HinP1I  
                                                          ~~~~   
                                                           DpnII 
                                                              ~~ 
             DdeI                                           DpnI 
             ~~~~~                                            ~~ 
                BmyI                                      CfoI   
               ~~~~~~~                                    ~~~~   
                AspHI      AciI  DdeI                      BstUI 
               ~~~~~~~     ~~~~  ~~~~~                     ~~~~  
    1426  TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA 
          ATGGACTCGT GGGTCAGGCG GGACTCGTTT CTGGGGTTGC TCTTCGCGCT 
                      BsrI                                       
                      ~~~~~                                      

                                             ScrFI               
                                             ~~~~~               
                                             NciI                
                                             ~~~~~               
                                             HpaII               
                                             ~~~~                
                                             DsaV                
                                             ~~~~~               
                                       Fnu4HI    DpnII           
                                        ~~~~~~   ~~~~            
            NlaIII                       BsiYI                   
             ~~~~                     ~~~~~~~~~~~~               
          DpnIISau96I            MaeIII  AciI    DpnI            
          ~~    ~~~~~~            ~~~~~  ~~~~~   ~~~~            
          DpnI  AvaII    BpmI         AciI      AlwI      NlaIII 
          ~~    ~~~~~~  ~~~~~~        ~~~~      ~~~~~         ~~ 
    1476  TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCACGGCA 
          AGTGTACCAG GACGACCTCA AGCACTGGCG GCGGCCCTAG TGAGTGCCGT 

                     RsaI          
                     ~~~~       
                     BsrGI         
                   ~~~~~~~      
          NlaIIIAluICsp6I          
          ~~    ~~~~~~~~~       
    1526  TGGACGAGCT GTACAAGTAA 
          ACCTGCTCGA CATGTTCATT 


General Description
  molecule name: phGFP, CVU43284
  molecule length: 4145 bp
  fragment length: 720 bp
  from: 826
  to:   1545
Functional Map
  CDS (1 signal)
    hGFP, S65T
      Start: 826   End: 1545
      NCBI gi: 1151242
  Modified_base (1 signal)
    S65T
      Start: 1021  End: 1023
      
Restriction Map
  Acc65I (Asp718): 0 sites  G/GTACC
  AccI: 0 sites  GT/MKAC
  AccIII (BseAI, BspEI, MroI): 0 sites  T/CCGGA
  AciI: 8 sites  C/CGC
       N1: 1045 
       N2: 1086 
       N3: 1153 
       N4: 1192 
       N5: 1330 
       N6: 1443 
       N7: 1503 
       N8: 1506 
  AcsI (ApoI): 0 sites  R/AATTY
  AcyI (BsaHI): 0 sites  GR/CGYC
  AflII (BfrI): 0 sites  C/TTAAG
  AflIII: 0 sites  A/CRYGT
  AgeI (PinAI): 0 sites  A/CCGGT
  AluI: 8 sites  AG/CT
       N1: 847 
       N2: 880 
       N3: 952 
       N4: 985 
       N5: 1201 
       N6: 1249 
       N7: 1360 
       N8: 1534 
  Alw44I (ApaLI, SnoI): 0 sites  G/TGCAC
  AlwI: 2 sites  GGATCNNNN/NN
       N1: 1321 (on complementary strand)
       N2: 1520 
  AlwNI: 0 sites  CAGNNN/CTG
  AosI (AviII, FspI): 0 sites  TGC/GCA
  ApaI: 0 sites  GGGCC/C
  AscI: 0 sites  GG/CGCGCC
  AseI (AsnI): 0 sites  AT/TAAT
  Asp700 (XmnI): 0 sites  GAANN/NNTTC
  AspEI (Eam1105I): 0 sites  GACNNN/NNGTC
  AspHI (BsiHKAI, HgiAI): 1 site  GWGCW/C
       N1: 1436 
  AspI (Tth111I): 0 sites  GACN/NNGTC
  AsuII (BstBI, Csp45I, NspV, SfuI): 0 sites  TT/CGAA
  AvaI: 0 sites  C/YCGRG
  AvaII: 1 site  G/GWCC
       N1: 1483 
  AvrII (BlnI): 0 sites  C/CTAGG
  BamHI: 0 sites  G/GATCC
  BanI: 1 site  G/GYRCC
       N1: 862 
  BanII: 0 sites  GRGCY/C
  BbrPI (PmaCI, PmlI): 0 sites  CAC/GTG
  BbsI (BpuAI): 0 sites  GAAGACNN/NNNNN
  BbvI: 6 sites  GCAGCNNNNNNNN/NNNNN
       N1: 972 (on complementary strand)
       N2: 1078 
       N3: 1362 
       N4: 1369 
       N5: 1395 (on complementary strand)
       N6: 1398 (on complementary strand)
  BcgI: 1 site  CGANNNNNNTGCNNNNNNNNNNNN/N
       N1: 951 
  BclI: 0 sites  T/GATCA
  BfaI (MaeI): 0 sites  C/TAG
  BglI: 0 sites  GCCNNNN/NGGC
  BglII: 0 sites  A/GATCT
  BmyI (Bsp1286I): 4 sites  GDGCH/C
       N1: 867 
       N2: 996 
       N3: 1245 
       N4: 1436 
  BpmI: 2 sites  CTGGAGNNNNNNNNNNNNNNNN/N
       N1: 1271 
       N2: 1511 
  Bpu1102I (CelII, EspI): 0 sites  GC/TNAGC
  BsaAI: 0 sites  YAC/GTR
  BsaBI (MamI): 0 sites  GATNN/NNATC
  BsaI: 0 sites  GGTCTCN/NNNNN
  BsaJI: 5 sites  C/CNNGG
       N1: 855 
       N2: 995 
       N3: 1158 
       N4: 1182 
       N5: 1237 
  BsaWI: 0 sites  W/CCGGW
  BsgI: 3 sites  GTGCAGNNNNNNNNNNNNNNNN/N
       N1: 955 (on complementary strand)
       N2: 1052 
       N3: 1376 
  BsiEI (McrI): 0 sites  CGRY/CG
  BsiWI: 0 sites  C/GTACG
  BsiYI (BslI): 3 sites  CCNNNNN/NNGG
       N1: 996 
       N2: 1159 
       N3: 1509 
  BsmAI: 0 sites  GTCTCN/NNNNN
  BsmFI: 0 sites  GGGACNNNNNNNNNN/NNNNN
  BsmI: 0 sites  GAATGCN/N
  Bsp120I: 0 sites  G/GGCCC
  BspDI (ClaI): 0 sites  AT/CGAT
  BspHI (RcaI): 0 sites  T/CATGA
  BspMI: 0 sites  ACCTGCNNNN/NNNNN
  BspWI (MwoI): 5 sites  GCNNNNN/NNGC
       N1: 918 
       N2: 978 
       N3: 991 
       N4: 1035 
       N5: 1044 
  BsrBI: 0 sites  GAG/CGG
  BsrDI: 0 sites  GCAATGNN/N
  BsrFI (Cfr10I): 1 site  R/CCGGY
       N1: 977 
  BsrGI (SspBI): 1 site  T/GTACA
       N1: 1536 
  BsrI: 1 site  ACTGGN/N
       N1: 1438 (on complementary strand)
  BssHII: 0 sites  G/CGCGC
  Bst1107I: 0 sites  GTA/TAC
  BstEII: 0 sites  G/GTNACC
  BstNI (MvaI): 5 sites  CC/WGG
       N1: 872 
       N2: 997 
       N3: 1109 
       N4: 1184 
       N5: 1238 
  BstUI (FnuDII, MvnI, ThaI): 2 sites  CG/CG
       N1: 1155 
       N2: 1473 
  BstXI: 0 sites  CCANNNNN/NTGG
  BstYI (XhoII): 1 site  R/GATCY
       N1: 1326 
  Bsu36I: 0 sites  CC/TNAGG
  CfoI (HhaI): 3 sites  GCG/C
       N1: 1116 
       N2: 1157 
       N3: 1473 
  Csp6I: 2 sites  G/TAC
       N1: 1255 
       N2: 1537 
  DdeI: 2 sites  C/TNAG
       N1: 1430 
       N2: 1448 
  DpnI: 3 sites  GA/TC
       N1: 1328 
       N2: 1476 
       N3: 1514 
  DpnII (MboI, NdeII, Sau3AI): 3 sites  N/GATCN
       N1: 1326 
       N2: 1474 
       N3: 1512 
  DraI: 0 sites  TTT/AAA
  DraII (EcoO109I): 0 sites  RG/GNCCY
  DraIII: 0 sites  CACNNN/GTG
  DrdI: 0 sites  GACNNNN/NNGTC
  DsaI: 0 sites  C/CRYGG
  DsaV: 7 sites  N/CCNGGN
       N1: 854 
       N2: 870 
       N3: 995 
       N4: 1107 
       N5: 1182 
       N6: 1236 
       N7: 1508 
  EaeI: 2 sites  Y/GGCCR
       N1: 898 
       N2: 1287 
  EagI (EclXI, XmaIII): 0 sites  C/GGCCG
  EarI (Ksp632I): 0 sites  CTCTTCN/NNNN
  Ecl136II: 0 sites  GAG/CTC
  Eco47III: 0 sites  AGC/GCT
  Eco57I: 3 sites  CTGAAGNNNNNNNNNNNNNNNN/N
       N1: 980 
       N2: 1024 (on complementary strand)
       N3: 1223 
  EcoNI: 0 sites  CCTNN/NNNAGG
  EcoRI: 0 sites  G/AATTC
  EcoRII: 5 sites  N/CCWGGN
       N1: 870 
       N2: 995 
       N3: 1107 
       N4: 1182 
       N5: 1236 
  EcoRV: 0 sites  GAT/ATC
  Esp3I: 0 sites  CGTCTCN/NNNNN
  Fnu4HI (ItaI): 8 sites  GC/NGC
       N1: 986 
       N2: 1045 
       N3: 1067 
       N4: 1351 
       N5: 1358 
       N6: 1409 
       N7: 1412 
       N8: 1506 
  FokI: 2 sites  GGATGNNNNNNNNN/NNNNN
       N1: 854 (on complementary strand)
       N2: 1220 (on complementary strand)
  HaeII: 0 sites  RGCGC/Y
  HaeIII: 4 sites  GG/CC
       N1: 900 
       N2: 1000 
       N3: 1289 
       N4: 1401 
  HgaI: 0 sites  GACGCNNNNN/NNNNNN
  HincII (HindII): 0 sites  GTY/RAC
  HindIII: 0 sites  A/AGCTT
  HinfI: 0 sites  G/ANTC
  HinP1I: 3 sites  G/CGC
       N1: 1114 
       N2: 1155 
       N3: 1471 
  HpaI: 0 sites  GTT/AAC
  HpaII (MspI): 4 sites  C/CGG
       N1: 855 
       N2: 918 
       N3: 978 
       N4: 1509 
  HphI: 6 sites  GGTGANNNNNNNN/N
       N1: 841 
       N2: 844 (on complementary strand)
       N3: 1012 (on complementary strand)
       N4: 1174 
       N5: 1198 
       N6: 1327 
  KasI: 0 sites  G/GCGCC
  KpnI: 0 sites  GGTAC/C
  KspI (SacII): 0 sites  CCGC/GG
  MaeII: 3 sites  A/CGT
       N1: 891 
       N2: 1104 
       N3: 1275 
  MaeIII: 2 sites  N/GTNACN
       N1: 1009 
       N2: 1498 
  MboII: 4 sites  GAAGANNNNNNNN/N
       N1: 1069 (on complementary strand)
       N2: 1114 (on complementary strand)
       N3: 1117 (on complementary strand)
       N4: 1312 
  MfeI (MunI): 0 sites  C/AATTG
  MluI: 0 sites  A/CGCGT
  MluNI (MscI): 0 sites  TGG/CCA
  MnlI: 8 sites  CCTCNNNNNNN/N
       N1: 835 (on complementary strand)
       N2: 916 (on complementary strand)
       N3: 922 (on complementary strand)
       N4: 1016 
       N5: 1153 (on complementary strand)
       N6: 1165 (on complementary strand)
       N7: 1216 (on complementary strand)
       N8: 1336 (on complementary strand)
  MseI (Tru9I): 0 sites  T/TAA
  MslI: 5 sites  CAYNN/NNRTG
       N1: 827 
       N2: 857 
       N3: 1007 
       N4: 1184 
       N5: 1313 
  MspA1I: 0 sites  CMG/CKG
  NaeI: 0 sites  GCC/GGC
  NarI: 0 sites  GG/CGCC
  NciI: 2 sites  CC/SGG
       N1: 856 
       N2: 1510 
  NcoI: 0 sites  C/CATGG
  NdeI: 0 sites  CA/TATG
  NgoMI: 0 sites  G/CCGGC
  NheI: 0 sites  G/CTAGC
  NlaIII: 6 sites  NCATG/N
       N1: 829 
       N2: 1063 
       N3: 1093 
       N4: 1288 
       N5: 1483 
       N6: 1528 
  NlaIV: 2 sites  GGN/NCC
       N1: 864 
       N2: 1402 
  NotI: 0 sites  GC/GGCCGC
  NruI: 0 sites  TCG/CGA
  NsiI: 0 sites  ATGCA/T
  NspI: 0 sites  RCATG/Y
  PacI: 0 sites  TTAAT/TAA
  PaeR7I (XhoI): 0 sites  C/TCGAG
  PflMI (Van91I): 0 sites  CCANNNN/NTGG
  PleI: 0 sites  GAGTCNNNN/NN
  PmeI: 0 sites  GTTT/AAAC
  Ppu10I: 0 sites  A/TGCAT
  PpuMI: 0 sites  RG/GWCCY
  Psp1406I: 0 sites  AA/CGTT
  PstI: 0 sites  CTGCA/G
  PvuI: 0 sites  CGAT/CG
  PvuII: 0 sites  CAG/CTG
  RsaI: 2 sites  GT/AC
       N1: 1256 
       N2: 1538 
  RsrII: 0 sites  CG/GWCCG
  SacI: 0 sites  GAGCT/C
  SalI: 0 sites  G/TCGAC
  SapI: 0 sites  GCTCTTCN/NNNN
  Sau96I: 3 sites  G/GNCC
       N1: 999 
       N2: 1400 
       N3: 1483 
  ScaI: 0 sites  AGT/ACT
  ScrFI: 7 sites  CC/NGG
       N1: 856 
       N2: 872 
       N3: 997 
       N4: 1109 
       N5: 1184 
       N6: 1238 
       N7: 1510 
  SexAI: 0 sites  A/CCWGGT
  SfaNI: 4 sites  GCATCNNNNN/NNNNN
       N1: 925 (on complementary strand)
       N2: 1203 
       N3: 1218 
       N4: 1317 
  SfcI: 0 sites  C/TRYAG
  SfiI: 0 sites  GGCCNNNN/NGGCC
  SgrAI: 0 sites  CR/CCGGYG
  SmaI: 0 sites  CCC/GGG
  SnaBI: 0 sites  TAC/GTA
  SpeI: 0 sites  A/CTAGT
  SphI: 0 sites  GCATG/C
  SspI: 0 sites  AAT/ATT
  StyI: 0 sites  C/CWWGG
  SwaI: 0 sites  ATTT/AAAT
  TaqI: 5 sites  T/CGA
       N1: 876 
       N2: 1170 
       N3: 1197 
       N4: 1212 
       N5: 1341 
  TfiI: 0 sites  G/AWTC
  Tsp509I: 0 sites  N/AATTN
  XbaI: 0 sites  T/CTAGA
  XcmI: 0 sites  CCANNNNN/NNNNTGG
  XmaI: 0 sites  C/CCGGG
  AatI (StuI): 0 sites  AGG/CCT
  AatII: 0 sites  GACGT/C
Annotations



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