Restriction sites in GFP

INFMEDLAB mamocell at INET.UNI-C.DK
Tue Jul 16 07:23:28 EST 1996


This is the restrictionmap of GFP from pGFP (XXU17997B). GFP: 289-1005 bp.

This is a search for many restriction sites using the NTI vector 3.0
program.
					Gregers J. Gram, july 1996.


                                    BsrI                 Tsp509I 
                                    ~~~~~                      ~ 
                       MboII          BpmI        Tsp509I        
                       ~~~~~         ~~~~~~~       ~~~~~         
     289  ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA 
          TACTCATTTC CTCTTCTTGA AAAGTGACCT CAACAGGGTT AAGAACAACT 
                                              BsmFI              
                                              ~~~~~              

                                     Tsp509I                     
                                      ~~~~                       
          Tsp509IHphI    MseI   BmyI  AcsI                  HphI 
          ~~~    ~~~~~~  ~~~~ ~~~~~~~~~~~~~~                ~~~~ 
     339  ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 
          TAATCTACCA CTACAATTAC CCGTGTTTAA AAGACAGTCA CCTCTCCCAC 
                                                         MnlI    
                                                         ~~~~    

                                             Tsp509I             
                                              ~~~~               
            HphI                           MseI                  
            ~~~~~                          ~~~~                  
          HphI                                AcsI          BsrI 
          ~                                  ~~~~~~          ~~~ 
     389  AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT 
          TTCCACTACG TTGTATGCCT TTTGAATGGG AATTTAAATA AACGTGATGA 
               SfaNI                                             
               ~~~~~                                             

                          NlaIII                                 
                           ~~~~                                  
                           NcoI                                  
                          ~~~~~~~                                
                              MluNI                              
                             ~~~~~~~                             
                             HaeIII                              
                              ~~~~~                              
                              EaeI                               
                             ~~~~~~~                             
                           DsaI                                  
                          ~~~~~~~                                
                           BsaJI                                 
                          ~~~~~~~                                
          BsrI             StyI          MaeIII                  
          ~~              ~~~~~~~          ~~~~~                 
     439  GGAAAACTAC CTGTTCCATG GCCAACACTT GTCACTACTT TCTCTTATGG 
          CCTTTTGATG GACAAGGTAC CGGTTGTGAA CAGTGATGAA AGAGAATACC 

                                     DpnII                       
                                     ~~~~                        
                                     DpnI                        
                                     ~~~~                        
                                         NdeI       NlaIII       
                                        ~~~~~~~       ~~~~       
     489  TGTTCAATGC TTTTCAAGAT ACCCAGATCA TATGAAACGG CATGACTTTT 
          ACAAGTTACG AAAAGTTCTA TGGGTCTAGT ATACTTTGCC GTACTGAAAA 

                                      RsaI                       
                                      ~~~~                       
                                      Csp6I                      
                                      ~~~~                       
                   NlaIII             BsrGI                      
                     ~~~~            ~~~~~~~                     
     539  TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC 
          AGTTCTCACG GTACGGGCTT CCAATACATG TCCTTTCTTG ATATAAAAAG 

                                   MaeII                         
                                   ~~~~                          
                                   BsaAI                         
                                  ~~~~~~                         
                                   BbrPI                         
                                  ~~~~~~                         
                                 AflIII Eco57I             HphI  
                                 ~~~~~~ ~~~~~~~            ~~~~~ 
     589  AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA 
          TTTCTACTGC CCTTGATGTT CTGTGCACGA CTTCAGTTCA AACTTCCACT 

                         TfiI                                    
                         ~~~~~                                   
                            TaqI                   MseI          
                            ~~~~~                  ~~~~~         
                  MseI   HinfI    MseI             DraI  MboII   
                  ~~~~~  ~~~~~    ~~~~            ~~~~~~~~~~~~   
     639  TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG 
          ATGGGAACAA TTATCTTAGC TCAATTTTCC ATAACTAAAA TTTCTTCTAC 

                                                            AccI 
                                                             ~~~ 
                            Tsp509I                     Bst1107I 
                             ~~~~~                           ~~~ 
     689  GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA 
          CTTTGTAAGA ACCTGTGTTT AACCTTATGT TGATATTGAG TGTGTTACAT 

                                                  MseI           
                                                  ~~~~~          
          AccINlaIII                    TfiI      HpaI           
          ~~~  ~~~~                     ~~~~~~   ~~~~~~~         
          Bst1107I                      HinfI    HincII  Tsp509I 
          ~~~                           ~~~~~~   ~~~~~~~     ~~~ 
     739  TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA ACTTCAAAAT 
          ATGTAGTACC GTCTGTTTGT TTTCTTACCT TAGTTTCAAT TGAAGTTTTA 

          Tsp509I       MboII               BfaI                 
          ~             ~~~~~               ~~~~                 
     789  TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC 
          ATCTGTGTTG TAACTTCTAC CTTCGCAAGT TGATCGTCTG GTAATAGTTG 

                   Tsp509I    Sau96I                             
                     ~~~~       ~~~~~                            
                     MfeI     HaeIII                             
                   ~~~~~~~      ~~~~                             
     839  AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC 
          TTTTATGAGG TTAACCGCTA CCGGGACAGG AAAATGGTCT GTTGGTAATG 

                                       DpnII                     
                                       ~~~~~                     
                                 TaqI  DpnI                      
                                 ~~~~  ~~~~~                     
                                 AsuII BstYI                DrdI 
                                ~~~~~~~~~~~~~              ~~~~~ 
     889  CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA 
          GACAGGTGTG TTAGACGGGA AAGCTTTCTA GGGTTGCTTT TCTCTCTGGT 
                                                         BsmAI   
                                                         ~~~~~   
                                       AlwI               BsaI   
                                       ~~~~~~            ~~~~~~  

                                   MspA1I                        
                                   ~~~~~~                        
            Sau96I                  Fnu4HI                       
             ~~~~~                   ~~~~~                       
          NlaIII                     AlwNI                       
          ~~~~                     ~~~~~~~~~~                    
          DrdI               MaeIIIAluI                   NlaIII 
          ~~~~~~~             ~~~~~~~~~~                   ~~~~  
             AvaII                  PvuII           NlaIII  FokI 
             ~~~~~                 ~~~~~~             ~~~~    ~~ 
     939  CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 
          GTACCAGGAA GAACTCAAAC ATTGTCGACG ACCCTAATGT GTACCGTACC 
                                     BbvI                        
                                     ~~~~~                       

          FokI                  
          ~~~               
     989  ATGAACTATA CAAATAG
          TACTTGATAT GTTTATC



General Description
  molecule name: XXU17997B
  molecule length: 3344 bp
  fragment length: 717 bp
  from: 289
  to:   1005
Restriction Map
  Acc65I (Asp718): 0 sites  G/GTACC
  AccI: 1 site  GT/MKAC
       N1: 738 
  AccIII (BseAI, BspEI, MroI): 0 sites  T/CCGGA
  AciI: 0 sites  C/CGC
  AcsI (ApoI): 2 sites  R/AATTY
       N1: 365 
       N2: 422 
  AcyI (BsaHI): 0 sites  GR/CGYC
  AflII (BfrI): 0 sites  C/TTAAG
  AflIII: 1 site  A/CRYGT
       N1: 611 
  AgeI (PinAI): 0 sites  A/CCGGT
  AluI: 1 site  AG/CT
       N1: 965 
  Alw44I (ApaLI, SnoI): 0 sites  G/TGCAC
  AlwI: 1 site  GGATCNNNN/NN
       N1: 911 (on complementary strand)
  AlwNI: 1 site  CAGNNN/CTG
       N1: 968 
  AosI (AviII, FspI): 0 sites  TGC/GCA
  ApaI: 0 sites  GGGCC/C
  AscI: 0 sites  GG/CGCGCC
  AseI (AsnI): 0 sites  AT/TAAT
  Asp700 (XmnI): 0 sites  GAANN/NNTTC
  AspEI (Eam1105I): 0 sites  GACNNN/NNGTC
  AspHI (BsiHKAI, HgiAI): 0 sites  GWGCW/C
  AspI (Tth111I): 0 sites  GACN/NNGTC
  AsuII (BstBI, Csp45I, NspV, SfuI): 1 site  TT/CGAA
       N1: 911 
  AvaI: 0 sites  C/YCGRG
  AvaII: 1 site  G/GWCC
       N1: 943 
  AvrII (BlnI): 0 sites  C/CTAGG
  BamHI: 0 sites  G/GATCC
  BanI: 0 sites  G/GYRCC
  BanII: 0 sites  GRGCY/C
  BbrPI (PmaCI, PmlI): 1 site  CAC/GTG
       N1: 614 
  BbsI (BpuAI): 0 sites  GAAGACNN/NNNNN
  BbvI: 1 site  GCAGCNNNNNNNN/NNNNN
       N1: 952 (on complementary strand)
  BcgI: 0 sites  CGANNNNNNTGCNNNNNNNNNNNN/N
  BclI: 0 sites  T/GATCA
  BfaI (MaeI): 1 site  C/TAG
       N1: 821 
  BglI: 0 sites  GCCNNNN/NGGC
  BglII: 0 sites  A/GATCT
  BmyI (Bsp1286I): 1 site  GDGCH/C
       N1: 363 
  BpmI: 1 site  CTGGAGNNNNNNNNNNNNNNNN/N
       N1: 336 
  Bpu1102I (CelII, EspI): 0 sites  GC/TNAGC
  BsaAI: 1 site  YAC/GTR
       N1: 614 
  BsaBI (MamI): 0 sites  GATNN/NNATC
  BsaI: 1 site  GGTCTCN/NNNNN
       N1: 927 (on complementary strand)
  BsaJI: 1 site  C/CNNGG
       N1: 455 
  BsaWI: 0 sites  W/CCGGW
  BsgI: 0 sites  GTGCAGNNNNNNNNNNNNNNNN/N
  BsiEI (McrI): 0 sites  CGRY/CG
  BsiWI: 0 sites  C/GTACG
  BsiYI (BslI): 0 sites  CCNNNNN/NNGG
  BsmAI: 1 site  GTCTCN/NNNNN
       N1: 927 (on complementary strand)
  BsmFI: 1 site  GGGACNNNNNNNNNN/NNNNN
       N1: 308 (on complementary strand)
  BsmI: 0 sites  GAATGCN/N
  Bsp120I: 0 sites  G/GGCCC
  BspHI (RcaI): 0 sites  T/CATGA
  BspMI: 0 sites  ACCTGCNNNN/NNNNN
  BspWI (MwoI): 0 sites  GCNNNNN/NNGC
  BsrBI: 0 sites  GAG/CGG
  BsrDI: 0 sites  GCAATGNN/N
  BsrFI (Cfr10I): 0 sites  R/CCGGY
  BsrGI (SspBI): 1 site  T/GTACA
       N1: 565 
  BsrI: 2 sites  ACTGGN/N
       N1: 319 
       N2: 442 
  BssHII: 0 sites  G/CGCGC
  Bst1107I: 1 site  GTA/TAC
       N1: 739 
  BstEII: 0 sites  G/GTNACC
  BstNI (MvaI): 0 sites  CC/WGG
  BstUI (FnuDII, MvnI, ThaI): 0 sites  CG/CG
  BstXI: 0 sites  CCANNNNN/NTGG
  BstYI (XhoII): 1 site  R/GATCY
       N1: 916 
  Bsu36I: 0 sites  CC/TNAGG
  CfoI (HhaI): 0 sites  GCG/C
  ClaI (BspDI): 0 sites  AT/CGAT
  Csp6I: 1 site  G/TAC
       N1: 566 
  DdeI: 0 sites  C/TNAG
  DpnI: 2 sites  GA/TC
       N1: 516 
       N2: 918 
  DpnII (MboI, NdeII, Sau3AI): 2 sites  N/GATCN
       N1: 514 
       N2: 916 
  DraI: 1 site  TTT/AAA
       N1: 679 
  DraII (EcoO109I): 0 sites  RG/GNCCY
  DraIII: 0 sites  CACNNN/GTG
  DrdI: 1 site  GACNNNN/NNGTC
       N1: 941 
  DsaI: 1 site  C/CRYGG
       N1: 455 
  DsaV: 0 sites  N/CCNGGN
  EaeI: 1 site  Y/GGCCR
       N1: 458 
  EagI (EclXI, XmaIII): 0 sites  C/GGCCG
  EarI (Ksp632I): 0 sites  CTCTTCN/NNNN
  Ecl136II: 0 sites  GAG/CTC
  Eco47III: 0 sites  AGC/GCT
  Eco57I: 1 site  CTGAAGNNNNNNNNNNNNNNNN/N
       N1: 639 
  EcoNI: 0 sites  CCTNN/NNNAGG
  EcoRI: 0 sites  G/AATTC
  EcoRII: 0 sites  N/CCWGGN
  EcoRV: 0 sites  GAT/ATC
  Esp3I: 0 sites  CGTCTCN/NNNNN
  Fnu4HI (ItaI): 1 site  GC/NGC
       N1: 966 
  FokI: 1 site  GGATGNNNNNNNNN/NNNNN
       N1: 1001 
  HaeII: 0 sites  RGCGC/Y
  HaeIII: 2 sites  GG/CC
       N1: 460 
       N2: 861 
  HgaI: 0 sites  GACGCNNNNN/NNNNNN
  HincII (HindII): 1 site  GTY/RAC
       N1: 778 
  HindIII: 0 sites  A/AGCTT
  HinfI: 2 sites  G/ANTC
       N1: 654 
       N2: 768 
  HinP1I: 0 sites  G/CGC
  HpaI: 1 site  GTT/AAC
       N1: 778 
  HpaII (MspI): 0 sites  C/CGG
  HphI: 4 sites  GGTGANNNNNNNN/N
       N1: 359 
       N2: 398 
       N3: 404 
       N4: 647 
  KasI: 0 sites  G/GCGCC
  KpnI: 0 sites  GGTAC/C
  KspI (SacII): 0 sites  CCGC/GG
  MaeII: 1 site  A/CGT
       N1: 613 
  MaeIII: 2 sites  N/GTNACN
       N1: 469 
       N2: 958 
  MboII: 3 sites  GAAGANNNNNNNN/N
       N1: 314 
       N2: 695 
       N3: 815 
  MfeI (MunI): 1 site  C/AATTG
       N1: 849 
  MluI: 0 sites  A/CGCGT
  MluNI (MscI): 1 site  TGG/CCA
       N1: 460 
  MnlI: 1 site  CCTCNNNNNNN/N
       N1: 376 (on complementary strand)
  MseI (Tru9I): 6 sites  T/TAA
       N1: 354 
       N2: 420 
       N3: 648 
       N4: 662 
       N5: 678 
       N6: 777 
  MslI: 0 sites  CAYNN/NNRTG
  MspA1I: 1 site  CMG/CKG
       N1: 965 
  NaeI: 0 sites  GCC/GGC
  NarI: 0 sites  GG/CGCC
  NciI: 0 sites  CC/SGG
  NcoI: 1 site  C/CATGG
       N1: 455 
  NdeI: 1 site  CA/TATG
       N1: 519 
  NgoMI: 0 sites  G/CCGGC
  NheI: 0 sites  G/CTAGC
  NlaIII: 7 sites  NCATG/N
       N1: 459 
       N2: 533 
       N3: 553 
       N4: 748 
       N5: 943 
       N6: 983 
       N7: 988 
  NlaIV: 0 sites  GGN/NCC
  NotI: 0 sites  GC/GGCCGC
  NruI: 0 sites  TCG/CGA
  NsiI: 0 sites  ATGCA/T
  NspI: 0 sites  RCATG/Y
  PacI: 0 sites  TTAAT/TAA
  PflMI (Van91I): 0 sites  CCANNNN/NTGG
  PleI: 0 sites  GAGTCNNNN/NN
  PmeI: 0 sites  GTTT/AAAC
  Ppu10I: 0 sites  A/TGCAT
  PpuMI: 0 sites  RG/GWCCY
  Psp1406I: 0 sites  AA/CGTT
  PstI: 0 sites  CTGCA/G
  PvuI: 0 sites  CGAT/CG
  PvuII: 1 site  CAG/CTG
       N1: 965 
  RsaI: 1 site  GT/AC
       N1: 567 
  RsrII: 0 sites  CG/GWCCG
  SacI: 0 sites  GAGCT/C
  SalI: 0 sites  G/TCGAC
  SapI: 0 sites  GCTCTTCN/NNNN
  Sau96I: 2 sites  G/GNCC
       N1: 860 
       N2: 943 
  ScaI: 0 sites  AGT/ACT
  ScrFI: 0 sites  CC/NGG
  SexAI: 0 sites  A/CCWGGT
  SfaNI: 1 site  GCATCNNNNN/NNNNN
       N1: 385 (on complementary strand)
  SfcI: 0 sites  C/TRYAG
  SfiI: 0 sites  GGCCNNNN/NGGCC
  SgrAI: 0 sites  CR/CCGGYG
  SmaI: 0 sites  CCC/GGG
  SnaBI: 0 sites  TAC/GTA
  SpeI: 0 sites  A/CTAGT
  SphI: 0 sites  GCATG/C
  SspI: 0 sites  AAT/ATT
  StyI: 1 site  C/CWWGG
       N1: 455 
  SwaI: 0 sites  ATTT/AAAT
  TaqI: 2 sites  T/CGA
       N1: 657 
       N2: 911 
  TfiI: 2 sites  G/AWTC
       N1: 654 
       N2: 768 
  Tsp509I: 7 sites  N/AATTN
       N1: 327 
       N2: 338 
       N3: 365 
       N4: 422 
       N5: 707 
       N6: 786 
       N7: 849 
  XbaI: 0 sites  T/CTAGA
  XcmI: 0 sites  CCANNNNN/NNNNTGG
  XhoI (PaeR7I): 0 sites  C/TCGAG
  XmaI: 0 sites  C/CCGGG
  AatI (StuI): 0 sites  AGG/CCT
  AatII: 0 sites  GACGT/C
Annotations




More information about the Fluorpro mailing list