fixed gfp sections

GUTERMANK at HELIX.MGH.HARVARD.EDU GUTERMANK at HELIX.MGH.HARVARD.EDU
Fri Jul 26 13:25:48 EST 1996


Hi friends/colleagues,
>
> I will be injecting a GFP infected glioma line into rat brain and want to
> see it on tissue sections.  I plan to perfuse the animal with 4% paraformaldeh
yde -
> Does anyone know how thick to cut sections on the cryostat to visualize the
> fluorescent cells, also - how long does the fluorescence last?
>
> Any relevant info appreciated.
> Thanks for your help on my other question too.
>
> Karen Gutereman
> MGH
> "gutermank at helix.mgh.harvard.edu"
>



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