GFP expression in CHO

Christa Baumstark-Khan Christa.Baumstark-Khan at dlr.de
Tue Jul 30 06:12:02 EST 1996


Dear GFPlers
I try to transfect CHO cells with the Clontech pGFP-N1 vector using a
liposome transfection assay. So far we succeded in the experiment as
under selection conditions (G418, 1.5 microgram per ml medium) we got
realy nice colonies, surviving the procedure. So far, so good, but non
(realy none!!!) of the colonies gives any shine of green fluorescence.
We harvested such colonies and cells, which have not been transfected
in order to measure fluorescence output in a spectrofluorimeter.
Again, ther was not a glimpse of fluorescence. It was our 3rd
transfection with the same result. Something is wrong, but what is it?
Has anyone an idea?
Thanks
Christa


Dr. Christa Baumstark-Khan

DLR, Institut fuer Luft- und Raumfahrtmedizin
Linder Hoehe
51147 Koeln

Tel.: (49) 02203-601-3145
Fax:  (49) 02203-61970
e-mail christa.baumstark-khan at dlr.de



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